Peripheral Circulating Exosomal miRNAs Potentially Contribute to the Regulation of Molecular Signaling Networks in Aging

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dc.creatorZhang, Hongxia
dc.creatorJin, Kunlin
dc.creator.orcid0000-0002-1336-348X (Jin, Kunlin)
dc.creator.orcid0000-0003-1594-6707 (Zhang, Hongxia)
dc.date.accessioned2022-09-09T14:08:43Z
dc.date.available2022-09-09T14:08:43Z
dc.date.issued2020-03-11
dc.description.abstractPeople are living longer than ever. Consequently, they have a greater chance for developing a functional impairment or aging-related disease, such as a neurodegenerative disease, later in life. Thus, it is important to identify and understand mechanisms underlying aging as well as the potential for rejuvenation. Therefore, we used next-generation sequencing to identify differentially expressed microRNAs (miRNAs) in serum exosomes isolated from young (three-month-old) and old (22-month-old) rats and then used bioinformatics to explore candidate genes and aging-related pathways. We identified 2844 mRNAs and 68 miRNAs that were differentially expressed with age. TargetScan revealed that 19 of these miRNAs are predicated to target the 766 mRNAs. Pathways analysis revealed signaling components targeted by these miRNAs: mTOR, AMPK, eNOS, IGF, PTEN, p53, integrins, and growth hormone. In addition, the most frequently predicted target genes regulated by these miRNAs were EIF4EBP1, insulin receptor, PDK1, PTEN, paxillin, and IGF-1 receptor. These signaling pathways and target genes may play critical roles in regulating aging and lifespan, thereby validating our analysis. Understanding the causes of aging and the underlying mechanisms may lead to interventions that could reverse certain aging processes and slow development of aging-related diseases.
dc.description.sponsorshipAmerican Heart Association: 18PRE34020126; National Institutes of Health: 5R21-NS094859-02.
dc.identifier.citationZhang, H., & Jin, K. (2020). Peripheral Circulating Exosomal miRNAs Potentially Contribute to the Regulation of Molecular Signaling Networks in Aging. International journal of molecular sciences, 21(6), 1908. https://doi.org/10.3390/ijms21061908
dc.identifier.issn1422-0067
dc.identifier.issue6
dc.identifier.urihttps://hdl.handle.net/20.500.12503/31708
dc.identifier.volume21
dc.publisherMDPI
dc.relation.urihttps://doi.org/10.3390/ijms21061908
dc.rights.holder© 2020 by the authors.
dc.rights.licenseAttribution 4.0 International (CC BY 4.0)
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.sourceInternational Journal of Molecular Sciences
dc.subjectaging
dc.subjectaging-related disease
dc.subjectexosomes
dc.subjectfunctional enrichment analysis
dc.subjectingenuity pathway analysis
dc.subjectmiRNA-mRNA networks
dc.subjectserum
dc.subject.meshAging / genetics
dc.subject.meshAnimals
dc.subject.meshCirculating MicroRNA / genetics
dc.subject.meshExosomes / genetics
dc.subject.meshGene Expression Profiling / methods
dc.subject.meshGene Expression Regulation
dc.subject.meshGene Regulatory Networks
dc.subject.meshHigh-Throughput Nucleotide Sequencing
dc.subject.meshModels, Animal
dc.subject.meshRats
dc.subject.meshSequence Analysis, RNA
dc.subject.meshSignal Transduction
dc.titlePeripheral Circulating Exosomal miRNAs Potentially Contribute to the Regulation of Molecular Signaling Networks in Aging
dc.typeArticle
dc.type.materialtext

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