Sensitivity Comparison of an Organic Based DNA Extraction Method to a Silica Based DNA Extraction System Utilizing Carrier RNA and Effects of a Post-PCR Purification Process

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dc.contributor.advisorArthur Eisenberg
dc.contributor.committeeMemberJoseph Warren
dc.contributor.committeeMemberJohn Planz
dc.creatorNguyen, Chau
dc.date.accessioned2019-08-22T21:11:46Z
dc.date.available2019-08-22T21:11:46Z
dc.date.issued2008-12-01
dc.date.submitted2014-03-18T14:02:27-07:00
dc.description.abstractNguyen, Chau D., Sensitivity Comparison of an Organic Based DNA Extraction Method to a Silica Based DNA Extraction System Utilizing Carrier RNA and Effects of a Post-PCR Purification Process. Master of Science (Forensic Genetics), December 2008, 2008, 84 pp., 24 tables, 9 figures, References, 33 titles. Organic extraction from forensic samples has consistently produced high DNA yields. However, organic extraction is time-consuming and contains many steps where sample manipulation can occur. In this study, sensitivity of organic extraction method is compared to QIAamp DNA Investigator Kit, a silica based extraction utilizing carrier RNA. The results suggested that the QIAamp DNA Investigator Kit is just as sensitive as organic extraction method in obtaining partial and full DNA profiles. The QIAamp DNA Investigator Kit is less time-consuming than organic extraction and can be automated on the QIAcube. The study also studied the reliability of a post-PCR purification kit in obtaining a better DNA profile. The Qiagen MinElute PCR Purification Kit increased the number of loci detected in 65% of samples tested.
dc.format.mimetypeapplication/pdf
dc.identifier.urihttps://hdl.handle.net/20.500.12503/29145
dc.language.isoen
dc.provenance.legacyDownloads0
dc.subjectCell and Developmental Biology
dc.subjectEquipment and Supplies
dc.subjectGenetic Processes
dc.subjectGenetics
dc.subjectGenetics and Genomics
dc.subjectHealth Information Technology
dc.subjectInvestigative Techniques
dc.subjectLife Sciences
dc.subjectMedical Genetics
dc.subjectMedicine and Health Sciences
dc.subjectMolecular Genetics
dc.subjectOther Genetics and Genomics
dc.subjectSensitivity comparison
dc.subjectorganic based
dc.subjectsilica based
dc.subjectDNA extraction method
dc.subjectDNA extraction system
dc.subjectPost-PCR Purification Process
dc.subjectforensic samples
dc.subjectsample manipulation
dc.subjectQIAamp DNA Investigator Kit
dc.subjectQIAcube
dc.subjectQiagenMinElute
dc.titleSensitivity Comparison of an Organic Based DNA Extraction Method to a Silica Based DNA Extraction System Utilizing Carrier RNA and Effects of a Post-PCR Purification Process
dc.typeProfessional Report
dc.type.materialtext
thesis.degree.departmentGraduate School of Biomedical Sciences
thesis.degree.disciplineForensic Genetics
thesis.degree.grantorUniversity of North Texas Health Science Center at Fort Worth
thesis.degree.nameMaster of Science

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