Validation of the PowerPlex(R) Fusion 6C System and Alternate Robotic Methods on the Hamilton MICROLAB(R) STAR

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dc.contributor.advisorPlanz, John V.
dc.contributor.committeeMemberBarber, Robert C.
dc.contributor.committeeMemberZascavage, Roxanne R.
dc.creatorSmith, Briana N.
dc.date.accessioned2020-04-17T20:00:57Z
dc.date.available2020-04-17T20:00:57Z
dc.date.issued2020-05
dc.description.abstractInternal validations are done in forensic laboratories to ensure the methods and procedures are working as expected. For this internship practicum, I conducted an internal validation of Promega's PowerPlex(R) Fusion 6C System, as well as, a Quarter Plate Method for the Hamilton MICROLAB(R) STARlet Robotic System. This validation project was done for the Kansas Bureau of Investigation's DNA Databank Unit. This validation consisted of testing a PBS wash versus no wash, a sensitivity study, an injection time study, an analytical threshold study, a stochastic threshold study, a precision study a reproducibility study, and a contamination study. The results of these validation studies demonstrated that both the novel Quarter Plate Method that was created and Promega's PowerPlex(R) Fusion 6C System can be successfully used by the DNA Databank Unit for sample processing.
dc.format.mimetypeapplication/pdf
dc.identifier.urihttps://hdl.handle.net/20.500.12503/29836
dc.language.isoen
dc.subjectforensic STR loci/markers
dc.subjectquarter plate method
dc.subjectreference DNA samples
dc.subject.meshDatabases, Nucleic Acid / standards
dc.subject.meshDatabases, Nucleic Acid / instrumentation
dc.subject.meshForensic Genetics / instrumentation
dc.subject.meshValidation Study
dc.titleValidation of the PowerPlex(R) Fusion 6C System and Alternate Robotic Methods on the Hamilton MICROLAB(R) STAR
dc.typeThesis
dc.type.materialtext
thesis.degree.departmentGraduate School of Biomedical Sciences
thesis.degree.disciplineForensic Genetics
thesis.degree.grantorUniversity of North Texas Health Science Center at Fort Worth
thesis.degree.nameMaster of Science

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