The Evaluation of Different Collection Methods for the Optimum Recovery of DNA from Bloodstains on Various Surfaces

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dc.contributor.advisorArthur Eisenberg
dc.creatorStricklin, Sarah N
dc.date.accessioned2019-08-22T21:18:32Z
dc.date.available2019-08-22T21:18:32Z
dc.date.issued2013-05-01
dc.date.submitted2013-04-29T14:36:05-07:00
dc.description.abstractThis study compared the ability of cotton tip swabs and 4N6FLOQ™Swabs (Life Technologies, Carlsbad, CA) to collect dried bloodstains from various surfaces and recover the DNA transferred onto each swab. This study also examined whether swabs moistened with phosphate buffered saline (PBS) rather than distilled water (dH2O) provided any advantage in the recovery of DNA. The DNA yield; the quality of the Short Tandem Repeat (STR) profiles generated; and the ease of use were evaluated for the two swabs. The FLOQ™ swabs yielded the most DNA in the majority of the samples collected from each of the surfaces. There was no indication that PBS compared with dH2O provided any significant advantage in DNA yield and the quality of the STR profiles generated.
dc.format.mimetypeapplication/pdf
dc.identifier.urihttps://hdl.handle.net/20.500.12503/29233
dc.language.isoen
dc.provenance.legacyDownloads1200
dc.subjectGenetics
dc.subjectGenetics and Genomics
dc.subjectMedical Sciences
dc.subjectMedicine and Health Sciences
dc.subjectcotton swab
dc.subjectfloq swab
dc.subjectswab
dc.subjectdistilled water
dc.subjectphosphate buffered saline
dc.subjectbloodstains
dc.subjectforensic
dc.subjectflocked
dc.subject4n6floq swab
dc.titleThe Evaluation of Different Collection Methods for the Optimum Recovery of DNA from Bloodstains on Various Surfaces
dc.typeThesis
dc.type.materialtext
thesis.degree.departmentGraduate School of Biomedical Sciences
thesis.degree.disciplineForensic Genetics
thesis.degree.grantorUniversity of North Texas Health Science Center at Fort Worth
thesis.degree.nameMaster of Science

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