Exosomes Derived From Bone Mesenchymal Stem Cells Ameliorate Early Inflammatory Responses Following Traumatic Brain Injury

Simple item page
dc.creatorNi, Haoqi
dc.creatorYang, Su
dc.creatorSiaw-Debrah, Felix
dc.creatorHu, Jiangnan
dc.creatorWu, Ke
dc.creatorHe, Zibin
dc.creatorYang, Jianjing
dc.creatorPan, Sishi
dc.creatorLin, Xiao
dc.creatorYe, Haotuo
dc.creatorXu, Zhu
dc.creatorWang, Fan
dc.creatorJin, Kunlin
dc.creatorZhuge, Qichuan
dc.creatorHuang, Lijie
dc.creator.orcid0000-0002-1336-348X (Jin, Kunlin)
dc.date.accessioned2022-09-09T14:08:41Z
dc.date.available2022-09-09T14:08:41Z
dc.date.issued2019-01-24
dc.description.abstractTraumatic brain injury (TBI) is a leading cause of mortality and disability worldwide. Although treatment guidelines have been developed, no best treatment option or medicine for this condition exists. Recently, mesenchymal stem cells (MSCs)-derived exosomes have shown lots of promise for the treatment of brain disorders, with some results highlighting the neuroprotective effects through neurogenesis and angiogenesis after TBI. However, studies focusing on the role of exosomes in the early stages of neuroinflammation post-TBI are not sufficient. In this study, we investigated the role of bone mesenchymal stem cells (BMSCs)-exosomes in attenuating neuroinflammation at an early stage post-TBI and explored the potential regulatory neuroprotective mechanism. We administered 30 mug protein of BMSCs-exosomes or an equal volume of phosphate-buffered saline (PBS) via the retro-orbital route into C57BL/6 male mice 15 min after controlled cortical impact (CCI)-induced TBI. The results showed that the administration of BMSCs-exosomes reduced the lesion size and improved the neurobehavioral performance assessed by modified Neurological Severity Score (mNSS) and rotarod test. In addition, BMSCs-exosomes inhibited the expression of proapoptosis protein Bcl-2-associated X protein (BAX) and proinflammation cytokines, tumor necrosis factor-alpha (TNF-alpha) and interleukin (IL)-1beta, while enhancing the expression of the anti-apoptosis protein B-cell lymphoma 2 (BCL-2). Furthermore, BMSCs-exosomes modulated microglia/macrophage polarization by downregulating the expression of inducible nitric oxide synthase (INOS) and upregulating the expression of clusters of differentiation 206 (CD206) and arginase-1 (Arg1). In summary, our result shows that BMSCs-exosomes serve a neuroprotective function by inhibiting early neuroinflammation in TBI mice through modulating the polarization of microglia/macrophages. Further research into this may serve as a potential therapeutic strategy for the future treatment of TBI.
dc.description.sponsorshipThe work has been supported by National Natural Science Foundation of China (81771262), Zhejiang Health Science and Technology Project (2016RCA022), and Zhejiang Key Research and Development Project (2017C03027).
dc.identifier.citationNi, H., Yang, S., Siaw-Debrah, F., Hu, J., Wu, K., He, Z., Yang, J., Pan, S., Lin, X., Ye, H., Xu, Z., Wang, F., Jin, K., Zhuge, Q., & Huang, L. (2019). Exosomes Derived From Bone Mesenchymal Stem Cells Ameliorate Early Inflammatory Responses Following Traumatic Brain Injury. Frontiers in neuroscience, 13, 14. https://doi.org/10.3389/fnins.2019.00014
dc.identifier.issn1662-4548
dc.identifier.urihttps://hdl.handle.net/20.500.12503/31695
dc.identifier.volume13
dc.publisherFrontiers Media S.A.
dc.relation.urihttps://doi.org/10.3389/fnins.2019.00014
dc.rights.holderCopyright © 2019 Ni, Yang, Siaw-Debrah, Hu, Wu, He, Yang, Pan, Lin, Ye, Xu, Wang, Jin, Zhuge and Huang.
dc.rights.licenseAttribution 4.0 International (CC BY 4.0)
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.sourceFrontiers in Neuroscience
dc.subjectbone mesenchymal stem cells
dc.subjectexosomes
dc.subjectinflammation
dc.subjectmicroglia/macrophage
dc.subjectneuroprotection
dc.subjecttraumatic brain injury
dc.titleExosomes Derived From Bone Mesenchymal Stem Cells Ameliorate Early Inflammatory Responses Following Traumatic Brain Injury
dc.typeArticle
dc.type.materialtext

Files

Original bundle

Now showing 1 - 1 of 1
Thumbnail Image
Name:
10.3389_fnins.2019.00014.pdf
Size:
1.92 MB
Format:
Adobe Portable Document Format
Description:
full text article
Download

Collections

Publications -- Kunlin Jin