Metabolic Heterogeneity of Cerebral Cortical and Cerebellar Astrocytes

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dc.creatorSun, Yuanhong
dc.creatorWinters, Ali
dc.creatorWang, Linshu
dc.creatorChaudhari, Kiran
dc.creatorBerry, Raymond
dc.creatorTang, Christina
dc.creatorLiu, Ran
dc.creatorYang, Shaohua
dc.creator.orcid0000-0002-3882-0616 (Liu, Ran)
dc.creator.orcid0000-0002-0405-0887 (Yang, Shaohua)
dc.date.accessioned2023-02-23T16:06:53Z
dc.date.available2023-02-23T16:06:53Z
dc.date.issued2023-01-22
dc.description.abstractAstrocytes play critical roles in regulating neuronal synaptogenesis, maintaining blood-brain barrier integrity, and recycling neurotransmitters. Increasing numbers of studies have suggested astrocyte heterogeneity in morphology, gene profile, and function. However, metabolic phenotype of astrocytes in different brain regions have not been explored. In this paper, we investigated the metabolic signature of cortical and cerebellar astrocytes using primary astrocyte cultures. We observed that cortical astrocytes were larger than cerebellar astrocytes, whereas cerebellar astrocytes had more and longer processes than cortical astrocytes. Using a Seahorse extracellular flux analyzer, we demonstrated that cortical astrocytes had higher mitochondrial respiration and glycolysis than cerebellar astrocytes. Cerebellar astrocytes have lower spare capacity of mitochondrial respiration and glycolysis as compared with cortical astrocytes. Consistently, cortical astrocytes have higher mitochondrial oxidation and glycolysis-derived ATP content than cerebellar astrocytes. In addition, cerebellar astrocytes have a fuel preference for glutamine and fatty acid, whereas cortical astrocytes were more dependent on glucose to meet energy demands. Our study indicated that cortical and cerebellar astrocytes display distinct metabolic phenotypes. Future studies on astrocyte metabolic heterogeneity and brain function in aging and neurodegeneration may lead to better understanding of the role of astrocyte in brain aging and neurodegenerative disorders.
dc.description.sponsorshipThis research was funded by National Institutes of Health grants NS109583 (S.Y.) and the Cancer Prevention and Research Institute of Texas #RP210046 (R.B.).
dc.identifier.citationSun, Y., Winters, A., Wang, L., Chaudhari, K., Berry, R., Tang, C., Liu, R., & Yang, S. (2023). Metabolic Heterogeneity of Cerebral Cortical and Cerebellar Astrocytes. Life (Basel, Switzerland), 13(1), 184. https://doi.org/10.3390/life13010184
dc.identifier.issn2075-1729
dc.identifier.issue1
dc.identifier.urihttps://hdl.handle.net/20.500.12503/32048
dc.identifier.volume13
dc.publisherMDPI
dc.relation.urihttps://doi.org/10.3390/life13010184
dc.rights.holder© 2023 by the authors.
dc.rights.licenseAttribution 4.0 International (CC BY 4.0)
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.sourceLife
dc.subjectastrocytes
dc.subjectcerebellum
dc.subjectcortex
dc.subjectglycolysis rate
dc.subjectheterogeneity
dc.subjectmetabolism
dc.subjectmitochondrial respiration
dc.titleMetabolic Heterogeneity of Cerebral Cortical and Cerebellar Astrocytes
dc.typeArticle
dc.type.materialtext

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