Use of ATCC[R] MSA-2002[TM] for Validation of Extraction and Amplification Techniques in 16S Microbial Community Profiling

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dc.contributor.advisorAllen, Michael
dc.contributor.committeeMemberWarren, Joseph
dc.contributor.committeeMemberZhang, Yan
dc.contributor.committeeMemberMaddux, Scott D.
dc.creatorFoley, Brianna A.
dc.date.accessioned2019-09-26T19:52:27Z
dc.date.available2019-09-26T19:52:27Z
dc.date.issued2018-05
dc.description.abstractThe implementation of microbiome analysis is an emerging area of focus in forensic research. However, microbiome analysis is not well validated for use in forensic analyses and there is no standard protocol in place. In this research a comprehensive analysis of extraction and amplification techniques employed during investigation of microbial communities was performed using ATCC[R] MSA-2002[TM] as a mock microbial community. Comparison of DNA extraction protocols was performed followed by an analysis of commercially-available polymerases. Samples were pooled for sequencing of the V4 region of 16S ribosomal RNA gene using the Illumina MiSeq System, and subsequently analyzed for community composition. The results were compared with the known genomic data of the mock microbial community and statistical methods were employed to determine the extent of deviation.
dc.format.mimetypeapplication/pdf
dc.identifier.urihttps://hdl.handle.net/20.500.12503/29683
dc.language.isoen
dc.subject16S rRNA gene
dc.subjectATCC MSA-2002
dc.subjectIllumina MiSeq
dc.subjectMicrobial forensics
dc.subjectMicrobiome analysis
dc.subjectNext-generation sequencing
dc.subject.meshRNA, Ribosomal, 16S
dc.subject.meshMicrobiota
dc.subject.meshForensic Genetics
dc.subject.meshGenome, Microbial
dc.titleUse of ATCC[R] MSA-2002[TM] for Validation of Extraction and Amplification Techniques in 16S Microbial Community Profiling
dc.typeThesis
dc.type.materialtext
thesis.degree.departmentGraduate School of Biomedical Sciences
thesis.degree.disciplineBiomedical Sciences
thesis.degree.grantorUniversity of North Texas Health Science Center at Fort Worth
thesis.degree.nameMaster of Science

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