Internal Validation of Quantifiler Human Quantfilier Y Human male DNA Quantification Kits Using Applied Biosystems 7500 Real-Time PCR

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dc.contributor.advisorJoseph Warren
dc.contributor.committeeMemberArthur Eisenberg
dc.contributor.committeeMemberJohn Planz
dc.creatorJones, Brekeitrea Minyon
dc.date.accessioned2019-08-22T21:11:33Z
dc.date.available2019-08-22T21:11:33Z
dc.date.issued2004-08-01
dc.date.submitted2014-05-13T07:24:05-07:00
dc.description.abstractJones, Brekeitrea Minyon. Internal Validation of Quantifiler Human and Quantifiler Y Human DNA Quantification kits using the Applied Biosystems 7500 Real-Time PCR System. Master of Science (Forensic Genetics), August 2004, 36 pages, 4 figures, 8 tables, 18 references. Quantitation of DNA is an essential step in DNA testing and methods used to quantitate DNA must be human specific. Quantitation of DNA is also important because the PCR reaction performs optimally in a narrow concentration range. Applied Biosystems (Foster City, CA) has recently introduced two new DNA quantitation kits, Quantifiler Human and Quantifiler Y Human Male DNA Quantification kits, which quantify the total amount of amplifiable DNA present in a sample. Both kits are based on the 5’ nuclease assay and Taqman probe-based technology which allow for a more accurate result which will minimize further problems downstream. To validate the use of the Quantifiler kits, a series of studies were performed to determine the reliability and performance of the kits. Results demonstrate this quantitation method produces similar results to those obtained by the Quantiblot method. In addition, results demonstrate that this quantitation method is highly sensitive and yields consistent results.
dc.format.mimetypeapplication/pdf
dc.identifier.urihttps://hdl.handle.net/20.500.12503/29142
dc.language.isoen
dc.provenance.legacyDownloads0
dc.subjectCell and Developmental Biology
dc.subjectComputational Biology
dc.subjectEquipment and Supplies
dc.subjectForensic Science and Technology
dc.subjectGenetics
dc.subjectGenetics and Genomics
dc.subjectGenetic Structures
dc.subjectHealth Information Technology
dc.subjectInvestigative Techniques
dc.subjectLife Sciences
dc.subjectMedicine and Health Sciences
dc.subjectOther Genetics and Genomics
dc.subjectInternal validation
dc.subjectQuantifiler human
dc.subjectY Human DNA Quantification kits
dc.subjectApplied Biosystems 7500 Real-Time PCR System
dc.subjectDNA amplification
dc.subjectnuclease assay
dc.subjectTaqMan probe-based technology
dc.subjectquantification method
dc.titleInternal Validation of Quantifiler Human Quantfilier Y Human male DNA Quantification Kits Using Applied Biosystems 7500 Real-Time PCR
dc.typeProfessional Report
dc.type.materialtext
thesis.degree.departmentGraduate School of Biomedical Sciences
thesis.degree.disciplineCell Biology and Genetics
thesis.degree.grantorUniversity of North Texas Health Science Center at Fort Worth
thesis.degree.nameMaster of Science

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