Detecting and Quantifying Oxidative DNA Damage using MinION Nanopore Sequencing

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dc.contributor.advisorPhillips, Nicole
dc.contributor.committeeMemberPlanz, John
dc.contributor.committeeMemberAllen, Michael
dc.contributor.committeeMemberHe, Shaoqing
dc.creatorBlessing, Alexandra M.
dc.date.accessioned2019-09-26T19:52:26Z
dc.date.available2019-09-26T19:52:26Z
dc.date.issued2018-05
dc.description.abstractA common biomarker of damaged DNA, particularly mitochondrial DNA, 8-oxoguanine (8-oxoG) has been identified as a possible contributor to neurodegenerative disorders, such as Alzheimer's disease, Parkinson's disease, preeclampsia, as well as type 1 and type 2 diabetes. Numerous methods have been developed to detect oxidative damage within the genome, including but not limited to immunological techniques, quantitative-polymerase chain reaction (qPCR), and in situ imaging. This study explores nanopore sequencing using the MinION Nanopore (Oxford Nanopore Technologies, Oxford, UK) as a more sensitive method of 8-oxoguanine detection, providing proof-of-concept for model training as well as preliminary model development.
dc.format.mimetypeapplication/pdf
dc.identifier.urihttps://hdl.handle.net/20.500.12503/29681
dc.language.isoen
dc.subjectDNA
dc.subjectoxidative damage
dc.subjectnanopore
dc.subjectDNA sequencing
dc.subject8-oxoquanine
dc.subject.meshSequence Analysis, DNA, methods
dc.subject.meshNanopores
dc.subject.meshDNA, Mitochondrial
dc.subject.meshDNA Damage
dc.subject.meshOxidative Stress
dc.titleDetecting and Quantifying Oxidative DNA Damage using MinION Nanopore Sequencing
dc.typeThesis
dc.type.materialtext
thesis.degree.departmentGraduate School of Biomedical Sciences
thesis.degree.disciplineBiochemistry and Molecular Biology
thesis.degree.grantorUniversity of North Texas Health Science Center at Fort Worth
thesis.degree.nameMaster of Science

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