Unravelling Novel Roles of Salivary Exosomes in the Regulation of Human Corneal Stromal Cell Migration and Wound Healing

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dc.creatorEscandon, Paulina
dc.creatorLiu, Angela
dc.creatorNicholas, Sarah E.
dc.creatorKhan, Asher
dc.creatorRiaz, Kamran M.
dc.creatorKaramichos, Dimitrios
dc.creator.orcid0000-0002-8761-3824 (Karamichos, Dimitrios)
dc.date.accessioned2022-09-20T20:19:48Z
dc.date.available2022-09-20T20:19:48Z
dc.date.issued2022-04-14
dc.description.abstractSalivary exosomes have demonstrated vast therapeutic and diagnostic potential in numerous diseases. This study pioneers previously unexplored roles of SE in the context of corneal wound healing by utilizing primary corneal stromal cells from healthy (HCFs), type I diabetes mellitus (T1DMs), type II DM (T2DMs), and keratoconus (HKCs) subjects. Purified, healthy human SEs carrying tetraspanins CD9+, CD63+, and CD81+ were utilized. Scratch and cell migration assays were performed after 0, 6, 12, 24, and 48 h following SE stimulation (5 and 25 microg/mL). Significantly slower wound closure was observed at 6 and 12 h in HCFs with 5 mug/mL SE and T1DMs with 5 and 25 mug/mL SE. All wounds were closed by 24-hour, post-wounding. HKCs, T1DMs, and T2DMs with 25microg/mL SE exhibited a significant upregulation of cleaved vimentin compared to controls. Thrombospondin 1 was significantly upregulated in HCFs, HKCs, and T2DMs with 25 microg/mL SE. Lastly, HKCs, T1DMs, and T2DMs exhibited a significant downregulation of fibronectin with 25 mug/mL SE. Whether SEs can be utilized to clinical settings in restoring corneal defects is unknown. This is the first-ever study exploring the role of SEs in corneal wound healing. While the sample size was small, results are highly novel and provide a strong foundation for future studies.
dc.description.sponsorshipThe authors would like to acknowledge the following for their financial support: National Eye Institute, National Institutes of Health; (EY028888; D.K).
dc.identifier.citationEscandon, P., Liu, A., Nicholas, S. E., Khan, A., Riaz, K. M., & Karamichos, D. (2022). Unravelling Novel Roles of Salivary Exosomes in the Regulation of Human Corneal Stromal Cell Migration and Wound Healing. International journal of molecular sciences, 23(8), 4330. https://doi.org/10.3390/ijms23084330
dc.identifier.issn1422-0067
dc.identifier.issue8
dc.identifier.urihttps://hdl.handle.net/20.500.12503/31797
dc.identifier.volume23
dc.publisherMDPI
dc.relation.urihttps://doi.org/10.3390/ijms23084330
dc.rights.holder© 2022 by the authors.
dc.rights.licenseAttribution 4.0 International (CC BY 4.0)
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.sourceInternational Journal of Molecular Sciences
dc.subjectcell migration
dc.subjectcorneal wound healing
dc.subjectdiabetes mellitus
dc.subjectfibrosis
dc.subjectkeratoconus
dc.subjectocular diseases
dc.subjectsalivary exosomes
dc.subject.meshCell Movement
dc.subject.meshCornea / metabolism
dc.subject.meshCorneal Injuries / metabolism
dc.subject.meshExosomes
dc.subject.meshHumans
dc.subject.meshStromal Cells
dc.subject.meshWound Healing
dc.titleUnravelling Novel Roles of Salivary Exosomes in the Regulation of Human Corneal Stromal Cell Migration and Wound Healing
dc.typeArticle
dc.type.materialtext

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