STR Typing of Reference Samples with Rapid DNA technology

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dc.contributor.advisorBruce Budowle
dc.creatorMoore, Andrea B.
dc.date.accessioned2019-08-22T21:31:19Z
dc.date.available2019-08-22T21:31:19Z
dc.date.issued2014-05-01
dc.date.submitted2014-05-22T08:00:05-07:00
dc.description.abstractDNA typing can be a labor intensive and time-consuming process which, even in ideal situations, can take up to approximately one full day from collected swab to generated genotype profile. To expedite the process from sample-to-result, automated rapid DNA typing platforms have been developed. These turnkey systems offer the potential of reducing the time from reference sample collection to fully-interpreted profile in less than two hours. However, for full consideration of rapid technology, results should be of equal quality to that of currently accepted methodologies. This project tested the hypothesis that automated rapid STR genotyping platforms perform as well as standard STR typing methods for typing reference samples by comparing the ability of NetBio’s DNAscan™ automated rapid STR genotyping platform (NetBio, Inc., Waltham, MA) and standard STR typing methods to generate DNA profiles from reference samples. Multiple buccal swabs, collected from a number of individuals, were analyzed with the DNAscan instrument, an automated rapid STR genotyping platform that utilizes PowerPlex® 16 chemistry (Promega Corporation, Madison, WI). The rapid STR results were comparable with those obtained from the same individuals by standard STR genotyping processes also employing PowerPlex® 16 chemistry.
dc.format.mimetypeapplication/pdf
dc.identifier.urihttps://hdl.handle.net/20.500.12503/29394
dc.language.isoen
dc.provenance.legacyDownloads128
dc.subjectGenetics
dc.subjectGenetics and Genomics
dc.subjectMedical Genetics
dc.subjectSTR typing
dc.subjectRapid DNA Technology
dc.subjectReference Samples
dc.titleSTR Typing of Reference Samples with Rapid DNA technology
dc.typeThesis
dc.type.materialtext
thesis.degree.disciplineForensic Genetics
thesis.degree.grantorUniversity of North Texas Health Science Center at Fort Worth
thesis.degree.nameMaster of Science

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