Multiplex of INDELs for Human Identification Markers

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dc.contributor.advisorBobby L. LaRue
dc.contributor.committeeMemberPatricia A. Gwirtz
dc.contributor.committeeMemberKunlin Jin
dc.creatorSage, Kelly A.
dc.date.accessioned2019-08-22T21:49:40Z
dc.date.available2019-08-22T21:49:40Z
dc.date.issued2016-05-01
dc.date.submitted2016-08-05T09:06:06-07:00
dc.description.abstractForensic scientists commonly use short tandem repeat (STR) loci when comparing an evidentiary profile to that of a reference profile. In commercially available STR kits, the amplified products tend to range from 100- 500 base pairs (bp) in length. For genomic DNA of degraded biological samples, the fragments are usually 180-200bps or less. Therefore, degraded biological samples may not produce a full STR profile. Another viable option has been proposed to enable successful typing of some degraded DNA samples. Insertion/ deletion (INDEL) polymorphisms are intergenic regions of the genome in which amplified products can be smaller in length than most STRs. Using highly discriminating markers is desirable to distinguish individuals. A multiplex panel of human identification (HID) INDEL markers that can individualize people would be beneficial. This project tested the hypothesis that INDELs, which can be used to identify individuals with high discriminatory power, can be developed as a multiplex PCR approach. To test this hypothesis, primers were designed and multiplexed together to amplify specific INDELs that have been previously identified to be suitable for human identity testing purposes.
dc.format.mimetypeapplication/pdf
dc.identifier.urihttps://hdl.handle.net/20.500.12503/29611
dc.language.isoen
dc.provenance.legacyDownloads65
dc.subjectMedical Sciences
dc.subjectMedicine and Health Sciences
dc.subjectINDELs
dc.subjectHID
dc.subjectHuman Identification
dc.subjectForensics
dc.subjectMultiplex
dc.titleMultiplex of INDELs for Human Identification Markers
dc.typeThesis
dc.type.materialtext
thesis.degree.departmentGraduate School of Biomedical Sciences
thesis.degree.disciplineForensic Genetics
thesis.degree.grantorUniversity of North Texas Health Science Center at Fort Worth
thesis.degree.nameMaster of Science

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