Cell Ghost Coated Polymeric Nanoformulation For Brain Metastasized Triple Negative Breast Cancer




Van Treuren, Timothy
Kumar, Piyush
Ranjan, Amalendu
Chaudhary, Pankaj
Vishwanatha, Jamboor


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Background: Nanoparticle-mediated targeted delivery has become the buzzword in cancer therapy due to its small size and ease in modulation. Cell ghost derived from cancer cells that mimic the cellular environment can be used for specific targeting of tumor in personalized therapy. Purpose: The purpose of this study is to design drug delivery system for personalized medicine to check the brain metastasized Triple Negative Breast Cancer (TNBC). We speculate that cell ghost derived from the tumor of the cancer patient can also be used as a personalized treatment of metastatic cancer. Methods: Cell ghost isolated from brain metastasized TNBC cells was coated on polymeric nanoparticles. Dynamic Light Scattering & Zeta Size analyzer (Malvern Instruments, USA) were used to determine the hydrodynamic size & surface charge of the cell ghost coated nanoparticles(CGNP) respectively. SDS-PAGE was used for comparative analysis of proteins in the cell ghost & CGNP. The Cell uptake of the dye-loaded CGNP was studied using the confocal microscope (Zeiss microscope USA). Results: We have successfully formulated cell ghost coated polymeric nanoparticles (CGNP).The size & surface charge of the CGNP are in desirable range to cross the blood-brain barrier to target brain metastasized TNBC. The SDS-PAGE analysis confirmed that protein contents of cell ghost are stable in CGNP. Confocal microscopic image analysis showed that maximum cellular uptake of these nanoparticles by TNBC cell line. Conclusions: In summary, we concluded that cell ghost isolated from TNBC cells could be used as targeting agents for brain metastatic TNBC. These nanoparticles have maximum cellular uptake and retain the protein contents of cell ghost on nanoformulation infers its possible role in generating personalized medicine for the brain metastasized TNBC treatment.