Identification of Novel Genes Involved in Escherichia coli Biofilm Formation

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dc.contributor.advisorJerry Simecka
dc.contributor.committeeMemberMing-Chi Wu
dc.creatorDesPlas, Rebecca L.
dc.date.accessioned2019-08-22T21:08:36Z
dc.date.available2019-08-22T21:08:36Z
dc.date.issued2003-05-01
dc.date.submitted2013-12-13T10:07:53-08:00
dc.description.abstractDesPlas, Rebecca L., Identification of Novel Genes Involved in Escherichia coli Biofilm Formation. Master of Science (Microbiology), May 2003, 77 pp., 6 tables, 11 figures, references, 55 titles. Transposon mutagenesis using a miniTn10::camR transposon generated 800 random insertion mutants displaying altered biofilm phenotypes as compared to the parent strain, TRMG F/M. Transduction of the resistance marker confirmed approximately 150 biofilm mutants. Amplifications of the insertion sites, nucleotide sequencing and BLAST searches against E. coli K-12 genomic databases, identified118 of these sites. Many of the interrupted genes are not known to be associated with biofilm formation. Four mutations were transduced into E. coli K-12 MG1655, creating altered biofilm phenotypes. A plasmid clone of the nhaAR operon complemented the corresponding mutations. Results indicate that the genes identified in this study influence biofilm formation. However, further studies are needed to determine the degree of impact in a wild type strain background.
dc.format.mimetypeapplication/pdf
dc.identifier.urihttps://hdl.handle.net/20.500.12503/29103
dc.language.isoen
dc.provenance.legacyDownloads0
dc.subjectCell and Developmental Biology
dc.subjectGenetics
dc.subjectGenetics and Genomics
dc.subjectGenomics
dc.subjectLife Sciences
dc.subjectMedical Genetics
dc.subjectMedicine and Health Sciences
dc.subjectMolecular Genetics
dc.subjectOther Cell and Developmental Biology
dc.subjectOther Genetics and Genomics
dc.subjectNovel genes
dc.subjectidentification
dc.subjectEscherichia coli
dc.subjectBiofilm formation
dc.subjectmicrobiology
dc.subjecttransposon mutagenesis
dc.subjectminiTn10::camR transposon
dc.subjectamplification
dc.subjectgenomic databases
dc.subjectmutation
dc.subjectbiofilm phenotypes
dc.subjectwild type strain background
dc.titleIdentification of Novel Genes Involved in Escherichia coli Biofilm Formation
dc.typeThesis
dc.type.materialtext
thesis.degree.departmentGraduate School of Biomedical Sciences
thesis.degree.disciplineBiochemistry and Molecular Biology
thesis.degree.grantorUniversity of North Texas Health Science Center at Fort Worth
thesis.degree.nameMaster of Science

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