Cell line authentication and contamination assessment for human cell cultures

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dc.contributor.advisorArthur J. Eisenberg
dc.contributor.committeeMemberRhonda Roby
dc.contributor.committeeMemberJohn V. Planz
dc.creatorOrmos, Andrea
dc.date.accessioned2019-08-22T21:24:10Z
dc.date.available2019-08-22T21:24:10Z
dc.date.issued2015-05-01
dc.date.submitted2015-07-08T08:36:33-07:00
dc.description.abstractCell line authentication is an essential step in ensuring the integrity and reproducibility of biomedical research. The major contaminants in cell cultures are fungi, viruses, bacteria and contamination from other cell lines of the same or different species. Contaminants alter the physiology and properties of cells, compromising the results of experiments. In this study, an improved multiplex assay was developed, detecting mycoplasma and mouse cell line contamination, while performing DNA typing. The assay was tested on cell cultures, the reproducibility of the assay was verified, sample collection and procedures were optimized and limit of detection for contaminants were determined. A survey was conducted to assess the interest in an in-house cell line authentication and contamination assessment service.
dc.format.mimetypeapplication/pdf
dc.identifier.urihttps://hdl.handle.net/20.500.12503/29305
dc.language.isoen
dc.provenance.legacyDownloads468
dc.subjectCell Anatomy
dc.subjectClinical Epidemiology
dc.subjectDiagnosis
dc.subjectEpidemiology
dc.subjectForensic Science and Technology
dc.subjectInvestigative Techniques
dc.subjectLaboratory and Basic Science Research
dc.subjectMedical Sciences
dc.subjectMedicine and Health Sciences
dc.subjectcell line authentication
dc.subjectmycoplasma contamination
dc.titleCell line authentication and contamination assessment for human cell cultures
dc.typeThesis
dc.type.materialtext
thesis.degree.departmentGraduate School of Biomedical Sciences
thesis.degree.disciplineForensic Genetics
thesis.degree.grantorUniversity of North Texas Health Science Center at Fort Worth
thesis.degree.nameMaster of Science

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