Serum Deprivation Induces Apoptosis of Retinal Ganglion Cells Utilizing Mitochondrial Signaling Pathways

dc.contributor.advisorVictoria Rudick
dc.contributor.committeeMemberRaghu Krishnamoorthy
dc.contributor.committeeMemberGanesh Prasnna
dc.creatorCharles, Irma E.
dc.date.accessioned2019-08-22T21:17:35Z
dc.date.available2019-08-22T21:17:35Z
dc.date.issued2003-12-01
dc.date.submitted2014-05-02T09:00:05-07:00
dc.description.abstractCharles, Irma E., Serum Deprivation Induced Apoptosis of Retinal Ganglion Cells Utilizing Mitochondrial Signaling Pathways. Master of Science (Biomedical Sciences), December 2003, 90 pp., 10 illustrations. Apoptosis is the genetically regulated death of retinal ganglion cells (RGC) in which there is a blockade of retrograde transport. This blockade results in the loss of neurotrophic growth factors that are essential for the survival of the RGCs. This study uses several different techniques to determine mechanisms underlying apoptosis in rat RGCs deprived of growth factors. An established line of transformed RGC was subjected to serum deprivation for 2-6 days and compared to RGC cells maintained in 10% FBS to study the cellular changes that occur as a result of the treatments. The results show that serum deprivation for 48 hours resulted in a 50% cell loss due to apoptosis. Apoptotic death was associated with activation of caspases 3, 8, and 9 along with increased levels of Bax and death receptors 3 & 4. These results indicate that serum deprivation results in RGC death via mitochondrial and also extrinsic pathways.
dc.format.mimetypeapplication/pdf
dc.identifier.urihttps://hdl.handle.net/20.500.12503/29221
dc.language.isoen
dc.provenance.legacyDownloads0
dc.subjectCell and Developmental Biology
dc.subjectCell Biology
dc.subjectCells
dc.subjectCellular and Molecular Physiology
dc.subjectLife Sciences
dc.subjectMedical Cell Biology
dc.subjectMedical Sciences
dc.subjectMedicine and Health Sciences
dc.subjectOther Cell and Developmental Biology
dc.subjectSerum deprivation
dc.subjectapoptosis
dc.subjectretinal ganglion cells
dc.subjectmitochondrial signaling pathways
dc.subjectRGC
dc.subjectretrograde transport
dc.subjectneurotropic growth factors
dc.subjectcell survival
dc.subjectextrinsic pathways
dc.titleSerum Deprivation Induces Apoptosis of Retinal Ganglion Cells Utilizing Mitochondrial Signaling Pathways
dc.typeProfessional Report
dc.type.materialtext
thesis.degree.departmentGraduate School of Biomedical Sciences
thesis.degree.disciplineBiomedical Sciences
thesis.degree.grantorUniversity of North Texas Health Science Center at Fort Worth
thesis.degree.nameMaster of Science
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