Analysis of Low Copy Number DNA Using Profiler Plus at Increased Amplification Cycles and Modifications in Sample Injection Parameters
dc.contributor.advisor | Arthur Eisenberg | |
dc.contributor.committeeMember | John Planz | |
dc.contributor.committeeMember | Joseph Warren | |
dc.creator | Hynds, Jody Lynn | |
dc.date.accessioned | 2019-08-22T21:04:27Z | |
dc.date.available | 2019-08-22T21:04:27Z | |
dc.date.issued | 2003-08-01 | |
dc.date.submitted | 2014-05-13T07:24:05-07:00 | |
dc.description.abstract | There are many DNA testing techniques that can be utilized for samples with low quantities of DNA. Mitochondrial DNA testing is designed for successful DNA sequencing of hair shafts, degraded and burned samples. Newly developed SNP (single nucleotide polymorphisms) testing is also designed for the analysis of challenging samples. The increased interest in the analysis of low copy number DNA samples using STR testing is necessitated since the national database CODIS (Combined Data Index System) currently only accepts the DNA profiles analyzed with the 13 core STR loci. CODIS contains DNA profiles of evidence found at crime scenes, convicted offender and missing persons DNA profiles (4). The goal of this project is to develop methodologies to increase the success rate of LCN DNA samples using STR testing. The experimental design for this study involved the amplification of DNA isolated from buccal swabs using the Profiler Plus multiplex kit at two different DNA input quantities: 0/0156ng (15.6pg) and 0.0312ng (31.2pg). Four separate amplifications of these DNA samples were done at: 28, 30, 32 and 34 cycles. The manufacturer’s recommended cycle number for AmpFISTR Profiler Plus is 28 cycles. These samples were analyzed on both the ABI Prism 310 Genetic Analyzer and the ABI Prism 3100 Genetic Analyzer using OCD standard protocols for loading samples. The injection time and voltage were modified for each of the number of PCR cycles. The best combination of cycle number and injection parameters was chosen for the low copy number reproducibility study. | |
dc.format.mimetype | application/pdf | |
dc.identifier.uri | https://hdl.handle.net/20.500.12503/29047 | |
dc.language.iso | en | |
dc.provenance.legacyDownloads | 0 | |
dc.subject | Cell and Developmental Biology | |
dc.subject | Computational Biology | |
dc.subject | Equipment and Supplies | |
dc.subject | Forensic Science and Technology | |
dc.subject | Genetics | |
dc.subject | Genetics and Genomics | |
dc.subject | Genetic Structures | |
dc.subject | Health Information Technology | |
dc.subject | Investigative Techniques | |
dc.subject | Life Sciences | |
dc.subject | Medicine and Health Sciences | |
dc.subject | Other Genetics and Genomics | |
dc.subject | DNA testing techniques | |
dc.subject | mitochondrial DNA testing | |
dc.subject | degraded samples | |
dc.subject | SNP | |
dc.subject | testing | |
dc.subject | challenging samples | |
dc.subject | challenged samples | |
dc.subject | low copy number DNA | |
dc.subject | STR testing | |
dc.subject | buccal swabs | |
dc.subject | Profiler Plus multiplex kit | |
dc.subject | AmpFlSTR Profiler Plus | |
dc.subject | PCR cycles | |
dc.subject | amplifications | |
dc.title | Analysis of Low Copy Number DNA Using Profiler Plus at Increased Amplification Cycles and Modifications in Sample Injection Parameters | |
dc.type | Professional Report | |
dc.type.material | text | |
thesis.degree.department | Graduate School of Biomedical Sciences | |
thesis.degree.discipline | Cell Biology and Genetics | |
thesis.degree.grantor | University of North Texas Health Science Center at Fort Worth | |
thesis.degree.name | Master of Science |
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