Investigating the Effect of 17β-Estradiol on 24(S)-hydroxycholesterol Concentration in Brain Tissue

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dc.contributor.advisorProkai, Laszlo
dc.contributor.committeeMemberProkai-Tatrai, Katalin
dc.contributor.committeeMemberLacko, Andras G.
dc.contributor.committeeMemberWoerner, August E.
dc.creatorOaxaca, K. Celeste
dc.date.accessioned2022-05-27T15:34:55Z
dc.date.available2022-05-27T15:34:55Z
dc.date.issued2021-05
dc.description.abstractEstrogen, more specifically 17β-estradiol (E2), has been found to have neuroprotective effects in the brain. With Alzheimer's patients being comprised mainly of women, it is worthwhile to investigate the role that reproductive senescence plays in the development and progression of the disease due to decreased levels of estrogen. The purpose of this research was to develop a method for analyzing how estrogen affects the concentration of 24(S)-hydroxycholesterol (24S-OHC) in brain tissue. Ovariectomized (OVX) CD1 mice we subjected to daily injections of E2 for 5 days. Cholesterol and 24SOHC from hippocampal tissue were analyzed by gas chromatography-mass spectrometry (GC-MS) assay developed for the purpose, and their concentrations were compared between the OVX E2 treated and OVX control groups. No significant difference was found between the two groups; nevertheless, the assay developed during this study will be beneficial for further investigation of E2 as a potential modulator of cholesterol metabolism in future studies.
dc.format.mimetypeapplication/pdf
dc.identifier.urihttps://hdl.handle.net/20.500.12503/31155
dc.language.isoen
dc.subject17β-Estradiol
dc.subjectcholesterol
dc.subjectoxysterol
dc.subject24(S)-hydroxycholesterol
dc.subjectbrain
dc.subjectmetabolism
dc.subject.meshEstradiol
dc.subject.meshNeurodegenerative Diseases / drug therapy
dc.subject.meshNeuroprotective Agents
dc.titleInvestigating the Effect of 17β-Estradiol on 24(S)-hydroxycholesterol Concentration in Brain Tissue
dc.typeThesis
dc.type.materialtext
thesis.degree.departmentGraduate School of Biomedical Sciences
thesis.degree.disciplinePharmacology and Neuroscience
thesis.degree.grantorUniversity of North Texas Health Science Center at Fort Worth
thesis.degree.nameMaster of Science

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