Examining the skin and workplace microbiome following the return to the University of North Texas Health Science Center after self-isolation

Simple item page
dc.contributor.advisorAllen, Michael S.
dc.contributor.committeeMemberZhang, Yan
dc.contributor.committeeMemberZascavage, Roxanne R.
dc.contributor.committeeMemberRosales, Armando
dc.creatorKhichi, Ophelia J.
dc.date.accessioned2022-10-26T15:26:26Z
dc.date.available2022-10-26T15:26:26Z
dc.date.issued2021-08
dc.description.abstractThe human skin microbiome contains trillions of microbiomes that colonize the human body. It is unknown how social distancing and an increase in handwashing due to the COVID-19 pandemic affect the bacterial communities that reside on the human hands & fomites. In this research, bacterial swabs from individual's dominant hand, forearm of their dominant hand, and a fomite from the workplace environment were taken, and the resident microflora, i.e., the skin microbiome, was investigated. The DNA from the samples were extracted and amplified by PCR. Samples were then pooled for sequencing of the V4 region of the 16S ribosomal RNA gene using Illumina's MiSeq platform and subsequently analyzed for community composition. The results were compared against each other to determine how an individual and a fomite's microbiome shifted following their return to work. Furthermore, the results were used to determine if individual's microbiome became more similar to each other as they returned to work in the same building.
dc.format.mimetypeapplication/pdf
dc.identifier.urihttps://hdl.handle.net/20.500.12503/31890
dc.language.isoen
dc.subject16S rRNA
dc.subjectDNA
dc.subjectmicrobiome
dc.subjectMiSeq
dc.subjectNGS
dc.subjectPCR
dc.subject.meshMicrobiota
dc.subject.meshSequence Analysis, DNA
dc.subject.meshPolymerase Chain Reaction
dc.titleExamining the skin and workplace microbiome following the return to the University of North Texas Health Science Center after self-isolation
dc.typeThesis
dc.type.materialtext
thesis.degree.departmentSchool of Biomedical Sciences
thesis.degree.disciplineBiomedical Sciences
thesis.degree.grantorUniversity of North Texas Health Science Center at Fort Worth
thesis.degree.nameMaster of Science

Files

Original bundle

Now showing 1 - 1 of 1
Thumbnail Image
Name:
2021_08_gsbs_Khichi_Ophelia_thesis.pdf
Size:
5.51 MB
Format:
Adobe Portable Document Format
Description:
Download

Collections

School of Biomedical Sciences
Theses and Dissertations