The Role of Lipid Rafts and Membrane Androgen Receptors in Androgen's Neurotoxic Effects

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dc.creatorFadeyibi, Oluwadarasimi
dc.creatorRybalchenko, Nataliya
dc.creatorMabry, Steve
dc.creatorNguyen, Dianna H.
dc.creatorCunningham, Rebecca L.
dc.creator.orcid0000-0001-5984-5516 (Cunningham, Rebecca)
dc.date.accessioned2022-09-06T14:39:01Z
dc.date.available2022-09-06T14:39:01Z
dc.date.issued2022-02-21
dc.description.abstractSex differences have been observed in multiple oxidative stress-associated neurodegenerative diseases. Androgens, such as testosterone, can exacerbate oxidative stress through a membrane androgen receptor (mAR), AR45, localized to lipid rafts in the plasma membrane. The goal of this study is to determine if interfering with mAR localization to cholesterol-rich lipid rafts decreases androgen induced neurotoxicity under oxidative stress environments. We hypothesize that cholesterol-rich caveolar lipid rafts are necessary for androgens to induce oxidative stress generation in neurons via the mAR localized within the plasma membrane. Nystatin was used to sequester cholesterol and thus decrease cholesterol-rich caveolar lipid rafts in a neuronal cell line (N27 cells). Nystatin was applied prior to testosterone exposure in oxidatively stressed N27 cells. Cell viability, endocytosis, and protein analysis of oxidative stress, apoptosis, and mAR localization were conducted. Our results show that the loss of lipid rafts via cholesterol sequestering blocked androgen-induced oxidative stress in cells by decreasing the localization of mAR to caveolar lipid rafts.
dc.description.sponsorshipThis study was supported by National Institute of Health (NIH) R01 NS0091359 to R.L.C.
dc.identifier.citationFadeyibi, O., Rybalchenko, N., Mabry, S., Nguyen, D. H., & Cunningham, R. L. (2022). The Role of Lipid Rafts and Membrane Androgen Receptors in Androgen's Neurotoxic Effects. Journal of the Endocrine Society, 6(5), bvac030. https://doi.org/10.1210/jendso/bvac030
dc.identifier.issn2472-1972
dc.identifier.issue5
dc.identifier.urihttps://hdl.handle.net/20.500.12503/31667
dc.identifier.volume6
dc.publisherOxford University Press
dc.relation.urihttps://doi.org/10.1210/jendso/bvac030
dc.rights.holder© The Author(s) 2022.
dc.rights.licenseAttribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.sourceJournal of the Endocrine Society
dc.subjectAr45
dc.subjectapoptosis
dc.subjectaromatase
dc.subjectcaveolin-1
dc.subjectendocytosis
dc.subjectlipid rafts
dc.subjectmembrane androgen receptor
dc.subjectnystatin
dc.subjectoxidative stress
dc.subjectstatin
dc.subjecttestosterone
dc.titleThe Role of Lipid Rafts and Membrane Androgen Receptors in Androgen's Neurotoxic Effects
dc.typeArticle
dc.type.materialtext

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