Evaluation of Molecular Techniques Using a Synthetic Mitochondrial Genome

Simple item page
dc.contributor.advisorRhonda Roby
dc.creatorKoenig, Jessica L.
dc.date.accessioned2019-08-22T20:04:58Z
dc.date.available2019-08-22T20:04:58Z
dc.date.issued2014-05-01
dc.date.submitted2014-05-22T08:00:05-07:00
dc.description.abstractThe mitochondrion is responsible for the bulk of cellular energy production through the process of oxidative phosphorylation. The mitochondrial genome (mtGenome) is subject to a high mutation rate due to its proximity to reactive oxygen species produced in energy production. Over 250 pathogenic mutations have been characterized, and studies have demonstrated mtDNA variations at the cellular, tissue, and individual level. Some of the characterization techniques include long range PCR and sequencing. Sanger sequencing has been the gold standard, but next-generation sequencing technologies are now available. These methods may be evaluated using synthetic DNA of known base composition. This project utilizes the first synthetic mtGenome to optimize a LR PCR protocol and evaluate sequence quality using Sanger, MiSeq System, and Ion Personal Genome Machine System sequencing platforms.
dc.format.mimetypeapplication/pdf
dc.identifier.urihttps://hdl.handle.net/20.500.12503/27746
dc.language.isoen
dc.provenance.legacyDownloads116
dc.subjectGenetics
dc.subjectGenetics and Genomics
dc.subjectMedical Genetics
dc.subjectMitochondrial DNA
dc.subjectLong Range PCR
dc.subjectNext Generation Sequencing
dc.subjectGenetics
dc.subjectPCR
dc.titleEvaluation of Molecular Techniques Using a Synthetic Mitochondrial Genome
dc.typeThesis
dc.type.materialtext
thesis.degree.disciplineForensic Genetics
thesis.degree.grantorUniversity of North Texas Health Science Center at Fort Worth
thesis.degree.nameMaster of Science

Files

Original bundle

Now showing 1 - 1 of 1
Thumbnail Image
Name:
2014_05_gsbs_Koenig_Jessica_thesis.pdf
Size:
2.44 MB
Format:
Adobe Portable Document Format
Download

Collections

School of Biomedical Sciences
Theses and Dissertations