Internal Validation of Promega's Powerplex 16 System

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dc.contributor.advisorJohn Planz
dc.contributor.committeeMemberArthur Eisenberg
dc.contributor.committeeMemberJoseph Warren
dc.creatorCondel, Kevin R.
dc.date.accessioned2019-08-22T21:51:39Z
dc.date.available2019-08-22T21:51:39Z
dc.date.issued2005-08-01
dc.date.submitted2014-05-02T09:00:05-07:00
dc.description.abstractCondel, Kevin R., Internal Validation Study of Promega’s Powerplex 16 System. Master of Science (Forensic Genetics), August, 2005, 83 pp., 18 tables, 8 illustrations, bibliography, 14 titles. In the environment surrounding a court case, the United States Justice System requires that DNA evidence presented in a court of law be reliable, robust, and reproducible. For DNA laboratories to uphold these strict guidelines, all methods and instrumentation used in the lab must undergo validation studies. A validation study will measure the ability of the laboratory to perform a new method or use new instrumentation on a variety of samples before casework can be performed, ensuring that the method or instrumentation is indeed reliable, robust, and reproducible. This study consists of the validation of Promega’s PowerPlex 16 kit on the ABI 3100 Avant Genetic Analyzer instrumentation for Identigene, located in Houston, Texas. The validation consists of sensitivity, routine, mixture, non-probative, allelic ladder size precision, and matrix evaluations. Completion of these studies allow Identigene to begin performing casework using the PowerPlex 16 Kit. A validation study of the Takayama Reagant for Identigene was also performed. This procedure is used when a substance is presumed to be blood and the laboratory personnel want to confirm the presence of heme. The study consisted of a test determining the amount of reducing agent to add to the reagent, a dilution study, and substrate studies, and after these tests were performed indicated the ineffectiveness of the Takayama procedure for the purposes of Identigene.
dc.format.mimetypeapplication/pdf
dc.identifier.urihttps://hdl.handle.net/20.500.12503/29635
dc.language.isoen
dc.provenance.legacyDownloads0
dc.subjectCell and Developmental Biology
dc.subjectComputational Biology
dc.subjectEquipment and Supplies
dc.subjectForensic Science and Technology
dc.subjectGenetics
dc.subjectGenetics and Genomics
dc.subjectGenetic Structures
dc.subjectHealth Information Technology
dc.subjectInvestigative Techniques
dc.subjectLife Sciences
dc.subjectMedicine and Health Sciences
dc.subjectOther Genetics and Genomics
dc.subjectInternal validation study
dc.subjectPromega’s Powerplex 16 System
dc.subjectDNA evidence
dc.titleInternal Validation of Promega's Powerplex 16 System
dc.typeProfessional Report
dc.type.materialtext
thesis.degree.departmentGraduate School of Biomedical Sciences
thesis.degree.disciplineCell Biology and Genetics
thesis.degree.grantorUniversity of North Texas Health Science Center at Fort Worth
thesis.degree.nameMaster of Science

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