The role of lipid rafts in androgen's neurotoxic effects

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dc.contributor.advisorCunningham, Rebecca L.
dc.contributor.committeeMemberCunningham, J. Thomas
dc.contributor.committeeMemberSumien, Nathalie
dc.contributor.committeeMemberYan, Liang-Jun
dc.contributor.committeeMemberPark, InWoo
dc.creatorFadeyibi, Oluwadarasimi M.
dc.date.accessioned2022-10-26T15:26:26Z
dc.date.available2022-10-26T15:26:26Z
dc.date.issued2021-08
dc.description.abstractSex differences have been observed in multiple oxidative stress (OS) associated neurodegenerative diseases. Androgens, the major male sex hormone, through a membrane androgen receptor (mAR) localized to lipid rafts in the plasma membrane exacerbates OS. The goal of this study is to determine if interfering with mAR localization to cholesterol-rich lipid rafts decreases androgen induced neurotoxicity under OS environments. We hypothesize that cholesterol-rich caveolar lipid rafts are necessary for androgens to induce OS generation in neurons via the mAR localized within the plasma membrane. Nystatin was used to deplete cholesterol-rich caveolar lipid rafts in N27 cells prior to testosterone exposure in oxidative stressed N27 cells. Cell viability and protein analysis of OS, apoptosis, and mAR localization were conducted. Our results show that the loss of lipid rafts blocked androgen-induced OS in cells by decreasing the localization of mAR to caveolar lipid rafts. These results are consistent with our clinical findings that showed a relationship between hyperlipidemia and androgens on dementia in men but not women.
dc.format.mimetypeapplication/pdf
dc.identifier.urihttps://hdl.handle.net/20.500.12503/31888
dc.language.isoen
dc.subjectandrogen
dc.subjectlipid shafts
dc.subjectstatins
dc.subject.meshOxidative Stress
dc.subject.meshMembrane Microdomains
dc.subject.meshAndrogens
dc.titleThe role of lipid rafts in androgen's neurotoxic effects
dc.typeThesis
dc.type.materialtext
thesis.degree.departmentSchool of Biomedical Sciences
thesis.degree.disciplineBiomedical Sciences
thesis.degree.grantorUniversity of North Texas Health Science Center at Fort Worth
thesis.degree.nameMaster of Science

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