Differential Expression of LLT1, SLAM Receptors CS1 and 2B4 and NCR Receptors NKp46 and NKp30 in Pediatric Acute Lymphoblastic Leukemia (ALL)

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dc.creatorPowers, Sheila B.
dc.creatorAhmed, Nourhan G.
dc.creatorJose, Roslin
dc.creatorBrezgiel, Marissa
dc.creatorAryal, Subhash
dc.creatorBowman, W. Paul
dc.creatorMathew, Porunelloor A.
dc.creatorMathew, Stephen O.
dc.creator.orcid0000-0001-5784-224X (Mathew, Stephen O.)
dc.creator.orcid0000-0001-8137-0895 (Mathew, Porunelloor A.)
dc.date.accessioned2023-03-27T15:24:24Z
dc.date.available2023-03-27T15:24:24Z
dc.date.issued2023-02-26
dc.description.abstractAcute lymphoblastic leukemia (ALL) represents the most common pediatric cancer. Most patients (85%) develop B-cell ALL; however, T-cell ALL tends to be more aggressive. We have previously identified 2B4 (SLAMF4), CS1 (SLAMF7) and LLT1 (CLEC2D) that can activate or inhibit NK cells upon the interaction with their ligands. In this study, the expression of 2B4, CS1, LLT1, NKp30 and NKp46 was determined. The expression profiles of these immune receptors were analyzed in the peripheral blood mononuclear cells of B-ALL and T-ALL subjects by single-cell RNA sequencing data obtained from the St. Jude PeCan data portal that showed increased expression of LLT1 in B-ALL and T-ALL subjects. Whole blood was collected from 42 pediatric ALL subjects at diagnosis and post-induction chemotherapy and 20 healthy subjects, and expression was determined at the mRNA and cell surface protein level. A significant increase in cell surface LLT1 expression in T cells, monocytes and NK cells was observed. Increased expression of CS1 and NKp46 was observed on monocytes of ALL subjects at diagnosis. A decrease of LLT1, 2B4, CS1 and NKp46 on T cells of ALL subjects was also observed post-induction chemotherapy. Furthermore, mRNA data showed altered expression of receptors in ALL subjects pre- and post-induction chemotherapy treatment. The results indicate that the differential expression of the receptors/ligand may play a role in the T-cell- and NK-cell-mediated immune surveillance of pediatric ALL.
dc.description.sponsorshipThis work was supported by the Cancer Foundation of North Texas (CRFNT) with number (97313), Leukemia Texas Inc. (77313b), Institute for Cancer Research, UNTHSC (RI6062) and Team Connor Childhood Cancer Foundation.
dc.identifier.citationPowers, S. B., Ahmed, N. G., Jose, R., Brezgiel, M., Aryal, S., Bowman, W. P., Mathew, P. A., & Mathew, S. O. (2023). Differential Expression of LLT1, SLAM Receptors CS1 and 2B4 and NCR Receptors NKp46 and NKp30 in Pediatric Acute Lymphoblastic Leukemia (ALL). International journal of molecular sciences, 24(4), 3860. https://doi.org/10.3390/ijms24043860
dc.identifier.issn1422-0067
dc.identifier.issue4
dc.identifier.urihttps://hdl.handle.net/20.500.12503/32062
dc.identifier.volume24
dc.publisherMDPI
dc.relation.urihttps://doi.org/10.3390/ijms24043860
dc.rights.holder© 2023 by the authors.
dc.rights.licenseAttribution 4.0 International (CC BY 4.0)
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.sourceInternational Journal of Molecular Sciences
dc.subject2b4
dc.subjectCs1
dc.subjectLlt1
dc.subjectNKp30
dc.subjectNKp46
dc.subjectacute lymphoblastic leukemia
dc.subjectnatural killer cell
dc.subject.meshChild
dc.subject.meshHumans
dc.subject.meshSignaling Lymphocytic Activation Molecule Family Member 1 / metabolism
dc.subject.meshLeukocytes, Mononuclear / metabolism
dc.subject.meshPrecursor T-Cell Lymphoblastic Leukemia-Lymphoma / metabolism
dc.subject.meshReceptors, Immunologic / metabolism
dc.subject.meshKiller Cells, Natural
dc.subject.meshCarrier Proteins / metabolism
dc.titleDifferential Expression of LLT1, SLAM Receptors CS1 and 2B4 and NCR Receptors NKp46 and NKp30 in Pediatric Acute Lymphoblastic Leukemia (ALL)
dc.typeArticle
dc.type.materialtext

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