Tissue Transglutaminase in Glaucoma

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dc.contributor.advisorClark, Abbot F.
dc.contributor.committeeMemberReeves, Rustin E.
dc.contributor.committeeMemberSheedlo, Harold
dc.creatorTovar-Vidales, Tara
dc.date.accessioned2019-08-22T21:19:41Z
dc.date.available2019-08-22T21:19:41Z
dc.date.issued2008-08-01
dc.date.submitted2013-11-15T13:18:03-08:00
dc.description.abstractTovar-Vidales, Tara, Tissue Transglutaminase in Glaucoma. Doctor of Philosophy (Cell Biology and Genetics), August 2008; 113pp; 0 tables, 23 illustrations, 165 bibliography, 25 titles. Primary open-angle glaucoma (POAG) is the second leading cause of irreversible blindness worldwide. Elevated intraocular pressure (IOP) is the major risk factor for POAG and is due to resistance of aqueous humor (AH) outflow through the trabecular meshwork ™ and Schlemm’s canal. Transforming growth factor-beta2 (TGF-β2) is elevated in the aqueous humor of glaucomatous eyes compared to normal eyes. Thus, TGF-β2 may play a role in regulating IOP. Tissue transglutaminase (TGM2) is a member of the transglutaminase family involved in cross-linking ECM proteins. In POAG, there are increased cross-linked extracellular matrix proteins (ECM) in the TM, and therefore, may result in elevated AH outflow resistance and elevated IOP. In this study, we examined the differences in both protein expression and enzyme activity of TGM2 between normal and glaucomatous TM cells and tissues. The findings demonstrated the presence of TGM2 in normal and glaucomatous cultured TM cells. We also showed that glaucomatous cultured TM cells and tissues have elevated levels of TGM2. Thus, this data suggest that TGM2 may have a pathogenic role in elevated outflow resistance and elevated IOP. Second, we observed the induction of TGM2 by TGF-β1, β2, and β3 in cultured TM cells, suggesting TGF-β isoforms regulate TGM2 protein levels. Finally, we observed that R-Smads and O38 regulated TGM2 protein levels, suggesting TGF-β2 acts through both its canonical and non-canonical signaling pathway to regulate TGM2.
dc.format.mimetypeapplication/pdf
dc.identifier.urihttps://hdl.handle.net/20.500.12503/29248
dc.language.isoen
dc.provenance.legacyDownloads2
dc.subjectCell Anatomy
dc.subjectCell and Developmental Biology
dc.subjectCell Biology
dc.subjectDiseases
dc.subjectEye Diseases
dc.subjectLife Sciences
dc.subjectMedical Cell Biology
dc.subjectMedicine and Health Sciences
dc.subjectOphthalmology
dc.subjectOptometry
dc.subjectSense Organs
dc.subjectSkin and Connective Tissue Diseases
dc.subjectTissues
dc.subjectVision Science
dc.subjectTissue transglutaminase
dc.subjectglaucoma
dc.subjectprimary open angle glaucoma
dc.subjectelevated intraocular pressure
dc.subjectaqueous humor
dc.subjecttransforming
dc.titleTissue Transglutaminase in Glaucoma
dc.typeDissertation
dc.type.materialtext
thesis.degree.departmentGraduate School of Biomedical Sciences
thesis.degree.disciplineCell Biology and Genetics
thesis.degree.grantorUniversity of North Texas Health Science Center at Fort Worth
thesis.degree.nameDoctor of Philosophy

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