Presence of Androgen Receptor Variant in Neuronal Lipid Rafts

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dc.creatorGarza-Contreras, Jo
dc.creatorDuong, Phong
dc.creatorSnyder, Brina D.
dc.creatorSchreihofer, Derek A.
dc.creatorCunningham, Rebecca L.
dc.creator.orcid0000-0001-5984-5516 (Cunningham, Rebecca)
dc.creator.orcid0000-0001-6254-4941 (Snyder, Brina D.)
dc.date.accessioned2022-09-06T14:39:00Z
dc.date.available2022-09-06T14:39:00Z
dc.date.issued2017-08-29
dc.description.abstractFast, nongenomic androgen actions have been described in various cell types, including neurons. However, the receptor mediating this cell membrane-initiated rapid signaling remains unknown. This study found a putative androgen receptor splice variant in a dopaminergic N27 cell line and in several brain regions (substantia nigra pars compacta, entorhinal cortex, and hippocampus) from gonadally intact and gonadectomized (young and middle-aged) male rats. This putative splice variant protein has a molecular weight of 45 kDa and lacks an N-terminal domain, indicating it is homologous to the human AR45 splice variant. Interestingly, AR45 was highly expressed in all brain regions examined. In dopaminergic neurons, AR45 is localized to plasma membrane lipid rafts, a microdomain involved in cellular signaling. Further, AR45 protein interacts with membrane-associated G proteins Galphaq and Galphao. Neither age nor hormone levels altered AR45 expression in dopaminergic neurons. These results provide the first evidence of AR45 protein expression in the brain, specifically plasma membrane lipid rafts. AR45 presence in lipid rafts indicates that it may function as a membrane androgen receptor to mediate fast, nongenomic androgen actions.
dc.description.sponsorshipThis study was funded by the National Institute of Neurological Disorders and Stroke (R01 NS088514) to RLC.
dc.identifier.citationGarza-Contreras, J., Duong, P., Snyder, B. D., Schreihofer, D. A., & Cunningham, R. L. (2017). Presence of Androgen Receptor Variant in Neuronal Lipid Rafts. eNeuro, 4(4), ENEURO.0109-17.2017. https://doi.org/10.1523/ENEURO.0109-17.2017
dc.identifier.issn2373-2822
dc.identifier.issue4
dc.identifier.urihttps://hdl.handle.net/20.500.12503/31665
dc.identifier.volume4
dc.publisherSociety for Neuroscience
dc.relation.urihttps://doi.org/10.1523/ENEURO.0109-17.2017
dc.rights.holderCopyright © 2017 Garza-Contreras et al.
dc.rights.licenseAttribution 4.0 International (CC BY 4.0)
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.sourceeNeuro
dc.subjectAndrogen receptor
dc.subjectG proteins
dc.subjectcaveolin
dc.subjectlipid rafts
dc.subjectmembrane
dc.subjectsignaling
dc.subject.meshAging / metabolism
dc.subject.meshAnimals
dc.subject.meshCA1 Region, Hippocampal / metabolism
dc.subject.meshCell Line
dc.subject.meshEntorhinal Cortex / metabolism
dc.subject.meshGTP-Binding Protein alpha Subunits / metabolism
dc.subject.meshMale
dc.subject.meshMembrane Microdomains / metabolism
dc.subject.meshNeurons / metabolism
dc.subject.meshOrchiectomy
dc.subject.meshPars Compacta / metabolism
dc.subject.meshProtein Isoforms / metabolism
dc.subject.meshRNA, Messenger / metabolism
dc.subject.meshRats
dc.subject.meshReceptors, Androgen / genetics
dc.subject.meshReceptors, Androgen / metabolism
dc.subject.meshTestis / metabolism
dc.subject.meshTestosterone / administration & dosage
dc.subject.meshTestosterone / metabolism
dc.titlePresence of Androgen Receptor Variant in Neuronal Lipid Rafts
dc.typeArticle
dc.type.materialtype

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