DELAYED NEURONAL DEATH IN SWINE FOLLOWING CARDIAC ARREST AND RESUSCITATION

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dc.creatorNguyen, Anh Q.
dc.creatorCherry, Brandon H.
dc.creatorMyoung-Gwi, Ryou
dc.creatorWilliams, Arthur G.
dc.creatorHollrah, Roger
dc.creatorBaker, Charla L.
dc.creatorChoudhury, Gourav
dc.creatorOlivencia-Yurvati, Albert H.
dc.creatorMallet, Robert T.
dc.date.accessioned2019-08-22T19:46:52Z
dc.date.available2019-08-22T19:46:52Z
dc.date.issued2014-03
dc.date.submitted2014-02-03T12:27:09-08:00
dc.descriptionResearch Appreciation Day Award Winner - 2014 Texas College of Osteopathic Medicine Awards - 3rd Place Student Research Award
dc.description.abstractPurpose (a): Cardiac arrest, a leading cause of death in the U.S., kills >90% of its victims, and survivors often are disabled by permanent brain injury inflicted by ischemia-reperfusion. Purkinje cells of the cerebellum and CA1 neurons of the hippocampus are especially vulnerable to post-ischemic neuronal death. We tested the hypothesis that cardiac arrest in a swine model caused delayed neuronal death. Methods (b): Yorkshire swine (25-35 kg) were subjected to cardiac arrest-resuscitation (n = 9) or non-arrest sham (n = 5) protocols. Ventricular fibrillation was induced by electrical pacing. Precordial compressions (100/min) were given at 6-10 min arrest, and then sinus rhythm was restored with transthoracic countershocks. NaCl was infused iv at 0.1 mmol/kg/min during CPR and the first 60 min after return of spontaneous circulation (ROSC). At 7 d ROSC, brain regions were fixed in 4% paraformaldehyde and H&E stained. Results (c): More than 70% of the Purkinje cells were shrunken, lacked dendrites and displayed condensed cytoplasm at 7 d ROSC; in contrast, in shams the majority of Purkinje cells retained the characteristic thick dendrites and well-defined nuclei. Conclusions (d): Thus, cardiac arrest-resuscitation produced marked changes in cerebellar neurons evident 7d after acute insult.
dc.identifier.urihttps://hdl.handle.net/20.500.12503/26970
dc.language.isoen
dc.subjectcardiac arrest
dc.subjectneuroprotection
dc.subjectischemia-reperfusion
dc.titleDELAYED NEURONAL DEATH IN SWINE FOLLOWING CARDIAC ARREST AND RESUSCITATION
dc.typeposter
dc.type.materialtext

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