Approaches to mitochondrial genome sequencing using the oxford nanopore minion device

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dc.contributor.advisorJohn V. Planz
dc.contributor.committeeMemberRance E. Berg
dc.contributor.committeeMemberJ. Thomas Cunningham
dc.creatorThorson, Kelcie C.
dc.date.accessioned2019-08-22T21:50:20Z
dc.date.available2019-08-22T21:50:20Z
dc.date.issued2017-05-01
dc.date.submitted2017-05-12T08:54:07-07:00
dc.description.abstractCurrent DNA sequencing methods rely on polymerase chain reaction (PCR) to create sufficient copies of targeted DNA fragments to serve as a library. PCR and subsequent clean-up steps add considerable time and cost to the process and provide opportunity for introduction of amplification errors or contamination. The aim of this study was to develop a reliable method for mitochondrial genome sequencing sans PCR using the Oxford Nanopore MinION. Our hypothesis was that results generated from native DNA sequencing would be concordant to sequencing results generated from PCR-enriched libraries. Error-rates of 1.47% (enriched) and 1.26% (native) were observed when sequencing the control DNA. Consensus sequences generated from native and PCR-enriched libraries show an average of 98.7% identity between the two methods.
dc.format.mimetypeapplication/pdf
dc.identifier.urihttps://hdl.handle.net/20.500.12503/29619
dc.language.isoen
dc.provenance.legacyDownloads48
dc.subjectGenetic Phenomena
dc.subjectGenetic Structures
dc.subjectInvestigative Techniques
dc.subjectMitochondrial genome sequencing
dc.subjectOxford Nanopore
dc.subjectMinION
dc.titleApproaches to mitochondrial genome sequencing using the oxford nanopore minion device
dc.typeThesis
dc.type.materialtext
thesis.degree.departmentGraduate School of Biomedical Sciences
thesis.degree.disciplineForensic Genetics
thesis.degree.grantorUniversity of North Texas Health Science Center at Fort Worth
thesis.degree.nameMaster of Science

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