Assessment of Oxford Nanopore Technologies as a Sequencing Platform for Increased Taxonomic Resolution of Microbial Communities

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dc.contributor.advisorPlanz, John V.
dc.contributor.committeeMemberAllen, Michael S.
dc.contributor.committeeMemberRoane, Brandy M.
dc.contributor.committeeMemberZascavage, Roxanne R.
dc.creatorValenti, Kiana L.
dc.date.accessioned2020-06-01T14:02:39Z
dc.date.available2020-06-01T14:02:39Z
dc.date.issued2019-05
dc.description.abstractMicrobiome profiling for forensic identification is an emerging area of research. Profiling the microbiome of trace evidence for forensic cases will become more prevalent as more reliable, accurate, and efficient methodology is developed. Sequencing the V4 region of the 16S rRNA gene using second-generation sequencing methods has traditionally been the means of characterizing the microbiome of microbial samples, however this method often does not garner species-level resolution. In this study, the MinION[TM] device from Oxford Nanopore Technologies, an instrument capable of sequencing the entire length of the 16S rRNA gene was used. The main goal of this was to determine whether using the MinION[TM] device is a more viable means of obtaining deeper yet still accurate taxonomic resolution of a mixed microbial standard that contains species of bacteria that might be found in a casework sample.
dc.format.mimetypeapplication/pdf
dc.identifier.urihttps://hdl.handle.net/20.500.12503/29849
dc.language.isoen
dc.subject16S rRNA gene
dc.subjectMinION
dc.subjectOxford Nanopore Technologies
dc.subjecttaxonomic resolution
dc.titleAssessment of Oxford Nanopore Technologies as a Sequencing Platform for Increased Taxonomic Resolution of Microbial Communities
dc.typeThesis
dc.type.materialtext
thesis.degree.departmentGraduate School of Biomedical Sciences
thesis.degree.disciplineForensic Genetics
thesis.degree.grantorUniversity of North Texas Health Science Center at Fort Worth
thesis.degree.nameMaster of Science

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