Limb Ischemic Conditioning Improved Cognitive Deficits via eNOS-Dependent Augmentation of Angiogenesis after Chronic Cerebral Hypoperfusion in Rats

Simple item page
dc.creatorRen, Changhong
dc.creatorLi, Ning
dc.creatorLi, Sijie
dc.creatorHan, Rongrong
dc.creatorHuang, Qingjian
dc.creatorHu, Jiangnan
dc.creatorJin, Kunlin
dc.creatorJi, Xunming
dc.creator.orcid0000-0002-1336-348X (Jin, Kunlin)
dc.date.accessioned2022-09-09T14:08:42Z
dc.date.available2022-09-09T14:08:42Z
dc.date.issued2018-10-01
dc.description.abstractIntracranial and extracranial arterial stenosis, the primary cause of chronic cerebral hypoperfusion (CCH), is a critical reason for the pathogenesis of vascular dementia and Alzheimer's disease characterized by cognitive impairments. Our previous study demonstrated that limb remote ischemic conditioning (LRIC) improved cerebral perfusion in intracranial arterial stenosis patients. The current study aimed to test whether LRIC promotes angiogenesis and increases phosphorylated endothelial nitric oxide synthase (p-eNOS) activity in CCH rat model. Adult male Sprague-Dawley rats were randomly assigned to three different groups: sham group, bilateral carotid artery occlusion (2VO) group and 2VO+LRIC group. Cerebral Blood Flow (CBF) was measured with laser speckle contrast imager at 4 weeks. Cognitive testing was performed at four and six weeks after 2VO surgery. We demonstrated that LRIC treatment increased cerebral perfusion and improved the CCH induced spatial learning and memory impairment. Immunohistochemistry confirmed that LRIC prevented cell death in the CA1 region, and increased the number of vessels and angiogenesis in the hippocampus after 2VO. Western blot analysis shows that LRIC therapy significantly increased p-eNOS expression in the hippocampus when compared with 2VO rats. Moreover, eNOS inhibitor reduced the effect of LRIC on angiogenesis in the hippocampus and spatial learning and memory function. Our data suggested that LRIC promoted angiogenesis, which is mediated, in part, by eNOS/NO.
dc.description.sponsorshipThis work was supported by the National Key R&D Program of China (2017YFC1308400), Distinguished Professor of Cheung Kong Scholars Program (T2014251), Beijing Municipal Administration of Hospitals Clinical Medicine Development of Special Funding Support (ZYLX201706) and Natural Science Foundation of China (81573867).
dc.identifier.citationRen, C., Li, N., Li, S., Han, R., Huang, Q., Hu, J., Jin, K., & Ji, X. (2018). Limb Ischemic Conditioning Improved Cognitive Deficits via eNOS-Dependent Augmentation of Angiogenesis after Chronic Cerebral Hypoperfusion in Rats. Aging and disease, 9(5), 869-879. https://doi.org/10.14336/AD.2017.1106
dc.identifier.issn2152-5250
dc.identifier.issue5
dc.identifier.urihttps://hdl.handle.net/20.500.12503/31701
dc.identifier.volume9
dc.publisherJKL International
dc.relation.urihttps://doi.org/10.14336/AD.2017.1106
dc.rights.holder© 2017 Ren C et al.
dc.rights.licenseAttribution 4.0 International (CC BY 4.0)
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.sourceAging and Disease
dc.subjectNO
dc.subjectangiogenesis
dc.subjectchronic cerebral hypoperfusion
dc.subjecteNOS
dc.subjectlimb ischemic conditioning
dc.titleLimb Ischemic Conditioning Improved Cognitive Deficits via eNOS-Dependent Augmentation of Angiogenesis after Chronic Cerebral Hypoperfusion in Rats
dc.typeArticle
dc.type.materialtext

Files

Original bundle

Now showing 1 - 1 of 1
Thumbnail Image
Name:
10.14336_AD.2017.1106.pdf
Size:
888.48 KB
Format:
Adobe Portable Document Format
Description:
full text article
Download

Collections

Publications -- Kunlin Jin