Evaluation of Reconstituted High-Density Lipoprotein as a Drug Delivery System in Drug-Sensitive and Drug-Resistant Ovarian Cancer Cell Lines

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dc.contributor.advisorBorejdo, Julian
dc.contributor.committeeMemberMcConathy, Walter J.
dc.contributor.committeeMemberNair, Maya P.
dc.creatorButtreddy, Sabitha
dc.date.accessioned2019-08-22T21:41:49Z
dc.date.available2019-08-22T21:41:49Z
dc.date.issued2004-05-01
dc.date.submitted2014-05-01T11:24:05-07:00
dc.description.abstractThe purpose of this study was to perform uptake and efflux studies of ORB in drug-sensitive (OV1063) and drug-resistant (SK-OV-3 and OVCAR-3) cells. Experiments were carried out by delivering ORB to the cells with and without rHDL to compare the amount of ORB taken up and expelled by the respective cells. Because serum contains lipoproteins, the experiments were also done in the absence of serum to show the specific effect of the rHDL vehicle. Because the efflux pumps responsible for multi-drug resistance are overexpressed and amplified in resistant cells, the efflux of ORB should be higher in these cells compared to the drug-sensitive cell line, OV1063. But, since our rHDL system is designed to reduce drug resistance by releasing the drug directly inside the cell bypassing the efflux pumps, the efflux of ORB should be less when it is delivered with rHDL.
dc.format.mimetypeapplication/pdf
dc.identifier.urihttps://hdl.handle.net/20.500.12503/29519
dc.language.isoen
dc.provenance.legacyDownloads0
dc.subjectCell Anatomy
dc.subjectCell and Developmental Biology
dc.subjectCell Biology
dc.subjectCells
dc.subjectChemicals and Drugs
dc.subjectDevelopmental Biology
dc.subjectLife Sciences
dc.subjectMedical Cell Biology
dc.subjectMedicine and Health Sciences
dc.subjectOther Cell and Developmental Biology
dc.subjectPharmacology
dc.subjectToxicology
dc.subjectEfflux study
dc.subjectuptake study
dc.subjectORB
dc.subjectdrug-sensitive cells
dc.subjectdrug-resistant cells
dc.subjectserum
dc.subjectlipoproteins
dc.subjectrHDL system
dc.titleEvaluation of Reconstituted High-Density Lipoprotein as a Drug Delivery System in Drug-Sensitive and Drug-Resistant Ovarian Cancer Cell Lines
dc.typeProfessional Report
dc.type.materialtext
thesis.degree.departmentGraduate School of Biomedical Sciences
thesis.degree.disciplineBiotechnology
thesis.degree.grantorUniversity of North Texas Health Science Center at Fort Worth
thesis.degree.nameMaster of Science

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