Evaluation of SPEX6750 Freezer Mill Pulveration of Teeth for Use in Nuclear Casework at the Armed Forces DNA Identification Laboratory

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dc.contributor.advisorJohn Planz
dc.contributor.committeeMemberArthur Eisenberg
dc.contributor.committeeMemberJoseph Warren
dc.creatorBintrim, Kristin A.
dc.date.accessioned2019-08-22T20:10:01Z
dc.date.available2019-08-22T20:10:01Z
dc.date.issued2008-08-01
dc.date.submitted2014-05-01T11:24:05-07:00
dc.description.abstractBintrim, Kristin A., Validation of Freezer Mill Pulveration of Teeth for use in Nuclear Casework at the Armed Forces DNA Identification Laboratory. Master of Science (Forensic Genetics), August, 2008, 44 pp., 4 tables, 6 figures, bibliography, 27 titles. I completed a validation study at the Armed Forces DNA Identification Laboratory. It was an evaluation of freezer mill pulveration of teeth for use in nuclear casework. The teeth were cleaned using a bleach and sonication method and then ground using a freezer mill. DNA extraction was achieved by using silica based capture techniques. The DNA was quantified, amplified and evaluated using fragment analysis. Several different amounts of input tooth powder were attempted to increase the DNA yield. Qiagen MinElute produced higher DNA yields then Promega’s DNA IQ system for the small sample size tested. After reviewing the results it was concluded that the DNA was most likely highly degraded and therefore did not produce complete genetic profiles for the samples tested.
dc.format.mimetypeapplication/pdf
dc.identifier.urihttps://hdl.handle.net/20.500.12503/27957
dc.language.isoen
dc.provenance.legacyDownloads0
dc.subjectBiomedical Devices and Instrumentation
dc.subjectCell and Developmental Biology
dc.subjectCell Biology
dc.subjectCells
dc.subjectEquipment and Supplies
dc.subjectForensic Science and Technology
dc.subjectGenetics
dc.subjectGenetics and Genomics
dc.subjectGenetic Structures
dc.subjectLife Sciences
dc.subjectMedical Cell Biology
dc.subjectMedical Genetics
dc.subjectMedicine and Health Sciences
dc.subjectOther Cell and Developmental Biology
dc.subjectOther Genetics and Genomics
dc.subjectFreezer mill pulveration
dc.subjectteeth
dc.subjectnuclear casework
dc.subjectArmed Forces DNA Identification Laboratory
dc.subjectbleach
dc.subjectsonication method
dc.subjectsilica based capture techniques
dc.subjectfragment analysis
dc.subjectQiagen MinElute
dc.subjectDNA yield
dc.subjectPromega’s DNA IQ
dc.subjectdegraded sample
dc.subjectgenetic profiles
dc.titleEvaluation of SPEX6750 Freezer Mill Pulveration of Teeth for Use in Nuclear Casework at the Armed Forces DNA Identification Laboratory
dc.typeProfessional Report
dc.type.materialtext
thesis.degree.departmentGraduate School of Biomedical Sciences
thesis.degree.disciplineForensic Genetics
thesis.degree.grantorUniversity of North Texas Health Science Center at Fort Worth
thesis.degree.nameMaster of Science

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