Sequencing Long Amplicon Microsatellite Loci Using the Oxford Nanopore Technologies MinION[TM] Device

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dc.contributor.advisorPlanz, John V.
dc.contributor.committeeMemberZascavage, Roxanne R.
dc.contributor.committeeMemberPhillips, Nicole R.
dc.contributor.committeeMemberMenegaz, Rachel A.
dc.creatorHall, Courtney L.
dc.date.accessioned2020-06-01T14:02:37Z
dc.date.available2020-06-01T14:02:37Z
dc.date.issued2019-05
dc.description.abstractForensic DNA typing utilizes highly variable short tandem repeat (STR) markers to differentiate individuals. Despite the power and reliability of current techniques, sequence-level variations are masked in the length-based profiles generated. Nanopore sequencing has the ability to provide long-read data, allowing for accurate alignment and identification of single nucleotide polymorphisms (SNPs) within and around microsatellite loci. To evaluate the applicability of nanopore sequencing to forensically-relevant autosomal and Y chromosome markers, selected STRs and their flanking regions (~800 bp) were amplified using custom primer sets, barcoded by sample, and sequenced on the MinION[TM] device. High quality sequencing data were obtained for all 24 samples at the 45 STRs interrogated using a customized data analysis pipeline. This project sets the foundation for future development of STRs for potential forensic applications as well as biomedically-relevant regions.
dc.format.mimetypeapplication/pdf
dc.identifier.urihttps://hdl.handle.net/20.500.12503/29845
dc.language.isoen
dc.subjectforensic
dc.subjectMinION
dc.subjectnanopore
dc.subjectSNP
dc.subjectSTR
dc.titleSequencing Long Amplicon Microsatellite Loci Using the Oxford Nanopore Technologies MinION[TM] Device
dc.typeThesis
dc.type.materialtext
thesis.degree.departmentGraduate School of Biomedical Sciences
thesis.degree.disciplineForensic Genetics
thesis.degree.grantorUniversity of North Texas Health Science Center at Fort Worth
thesis.degree.nameMaster of Science

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