Morphological and Proliferative Changes that Occur in Rat Retinal Progenitor Cells Following Incubation with Retinoic Acid and RPE-Secreted Proteins

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dc.contributor.advisorRustin E. Reeves
dc.creatorHeath, Allison K.
dc.date.accessioned2019-08-22T20:24:17Z
dc.date.available2019-08-22T20:24:17Z
dc.date.issued2006-08-01
dc.date.submitted2014-02-05T06:30:05-08:00
dc.description.abstractHeath Allison K., Morphological and Proliferative Changes that Occur in Rat Retinal Progenitor Cells Following Incubation With Retinonoic Acid and RPE-Secreted Proteins. Master’s of Science (Cell Biology and Genetics), August 2006, 67 pp., 12 figures, bibliography. The principal objective of this research is to characterize virally-transformed rat retinal progenitor cells following stimulation by retinal pigment epithelial (RPE) cell secreted proteins and retinoic acid. Progenitor cells were isolated from explants of postnatal rat RPE cell in vitro. Isolated progenitor cells were cloned, analyzed by microscopy and proliferation bioassays, to determine if cell proliferation occurred. The isolated progenitor cells were analyzed for differentiation by Western blot analyses and immunocytochemistry. The rat progenitor cells cultured in RPE secreted proteins proliferated, but did not differentiate as shown by the presence of nestin and vimentin in these cells. Retinoic acid caused other progenitor cells to proliferate and differentiate, which is seen through the bioassays and Western blot analyses.
dc.format.mimetypeapplication/pdf
dc.identifier.urihttps://hdl.handle.net/20.500.12503/28325
dc.language.isoen
dc.provenance.legacyDownloads0
dc.subjectCell and Developmental Biology
dc.subjectCell Biology
dc.subjectCells
dc.subjectCellular and Molecular Physiology
dc.subjectLife Sciences
dc.subjectMedical Cell Biology
dc.subjectMedical Genetics
dc.subjectMedicine and Health Sciences
dc.subjectMicrobiology
dc.subjectOther Cell and Developmental Biology
dc.subjectVirology
dc.subjectMorphological
dc.subjectproliferative
dc.subjectchanges
dc.subjectrat
dc.subjectretinal progenitor cells
dc.subjectincubation
dc.subjectretinoic acid
dc.subjectRPE-secreted proteins
dc.subjectretinal pigment epithelial cells
dc.subjectproteins
dc.titleMorphological and Proliferative Changes that Occur in Rat Retinal Progenitor Cells Following Incubation with Retinoic Acid and RPE-Secreted Proteins
dc.typeThesis
dc.type.materialtext
thesis.degree.departmentGraduate School of Biomedical Sciences
thesis.degree.disciplineCell Biology and Genetics
thesis.degree.grantorUniversity of North Texas Health Science Center at Fort Worth
thesis.degree.nameMaster of Science

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