Validation of Four Multiplex SNP Panels for Forensic DNA Testing: An Assessment of the Sensitivity and Reproducibility of the SNaPshot Primer Extension Assay

Simple item page
dc.contributor.advisorJohn Planz
dc.contributor.committeeMemberArthur Eisenberg
dc.contributor.committeeMemberJoseph Warren
dc.creatorDutton, Kristi R.
dc.date.accessioned2019-08-22T21:52:26Z
dc.date.available2019-08-22T21:52:26Z
dc.date.issued2003-05-01
dc.date.submitted2014-05-05T12:18:05-07:00
dc.description.abstractOrchid Cellmark provided four proprietary multiplex SNP primer panels, each of which has been developed to identify 10-12 SNPs using a modified version of the SNaPshot Mutliplex protocol. The commercially available STR typing kits routinely used in forensic testing require an input of between 0.5 to 2ng of DNA. Orchid Cellmark has suggested using 2ng of DNA with each of their multiplex SNP primer panels. However, preliminary data has indicated that as little as 100pg of DNA can yield results with many of the SNP markers. Several methods of SNP detection exist. This project relied on the use of multiplex SNP extension primers in conjunction with the ABI SNaPshot Multiplex Kit (Applied Biosystems, Foster City, CA) to identify SNPs in the nuclear genome. Analysis of 50 or more SNPs would be very laborious if single-tube polymerase chain reaction (PCR) was used for sample testing. Multiplexing SNP primers to include 10-12 per reaction tube will increase the throughput of SNP analysis. The cost of analysis can also be reduced using multiplexes since the amount of reagents per SNP is decreased. This project determined if the SNaPshot extension assay used in conjunction with the Orchid Cellmark multiplex panels could accurately detect SNPs at quantities less than 2ng DNA on a capillary electrophoresis (CE) platform.
dc.format.mimetypeapplication/pdf
dc.identifier.urihttps://hdl.handle.net/20.500.12503/29644
dc.language.isoen
dc.provenance.legacyDownloads0
dc.subjectCell and Developmental Biology
dc.subjectComputational Biology
dc.subjectEquipment and Supplies
dc.subjectForensic Science and Technology
dc.subjectGenetics
dc.subjectGenetics and Genomics
dc.subjectGenetic Structures
dc.subjectHealth Information Technology
dc.subjectInvestigative Techniques
dc.subjectLife Sciences
dc.subjectMedicine and Health Sciences
dc.subjectOther Genetics and Genomics
dc.subjectMultiplex SNP primer panels
dc.subjectSNaPshot Multiplex protocol
dc.subjectOrchid Cellmark
dc.subjectDNA
dc.subjectABI SNaPshot Multiplex kit
dc.subjectSNPs
dc.subjectNuclear genome
dc.subjectPCR
dc.subjectpolymerase chain reaction
dc.subjectcapillary electrophoresis platform
dc.titleValidation of Four Multiplex SNP Panels for Forensic DNA Testing: An Assessment of the Sensitivity and Reproducibility of the SNaPshot Primer Extension Assay
dc.typeProfessional Report
dc.type.materialtext
thesis.degree.departmentGraduate School of Biomedical Sciences
thesis.degree.disciplineCell Biology and Genetics
thesis.degree.grantorUniversity of North Texas Health Science Center at Fort Worth
thesis.degree.nameMaster of Science

Files

Original bundle

Now showing 1 - 1 of 1
Thumbnail Image
Name:
Dutton_ValidationOfFourMultiplex.pdf
Size:
27.02 MB
Format:
Adobe Portable Document Format
Download

Collections

School of Biomedical Sciences
Theses and Dissertations