A Novel sRNA Member of the Carbon Storage Regulatory System of Escherichia Coli

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dc.contributor.advisorJerry Simecka
dc.creatorWeilbacher, Thomas
dc.date.accessioned2019-08-22T21:51:50Z
dc.date.available2019-08-22T21:51:50Z
dc.date.issued2002-12-01
dc.date.submitted2014-04-09T06:36:22-07:00
dc.description.abstractWeilbacher, Thomas S., A Novel sRNA Member of the Carbon Storage Regulatory System of Escherichi coli. Master of Science (Microbiology & Immunology), December, 2002, 57 pp., 2 tables, 12 illustrations, bibliography, 44 titles. Small untranslated RNAs (sRNAs) perform a variety of important functions in bacterial systems. The 245 nt sRNA of Escherichia coli K-12, CsrC, was uncovered using a genetic screen for genes that regulate glycogen biosynthesis. CsrC RNA binds multiple copies of CsrA, a protein that post-transcriptionally regulates central carbon flux, biofilm formation, and motility in E. coli. CsrC antagonizes the regulatory effects of CsrA, presumably by sequestering this protein. The discovery of CsrC is intriguing, in that a similar sRNA, CsrB, performs essentially the same function. Both of these sRNAs possess similar imperfect repeat sequences (18 in CsrB, 9 in CsrC), primarily localized in the loops of predicted hairpins, which may serve as CsrA binding elements. Transcription of csrC increases as the culture approaches the stationary phase of growth and is activated by CsrA and the response regulator UvrY. Complementation and in vitro transcription-translation experiments reveal that CsrA effects on csrC are mediated indirectly, through UvrY. Because CsrB and CsrC antagonize the activity of CsrA and are dependent on CsrA for their synthesis, a csrB null mutation causes a modest compensatory increase in CsrC levels and vice versa. An updated model for the signaling circuitry of the Csr system is discussed.
dc.format.mimetypeapplication/pdf
dc.identifier.urihttps://hdl.handle.net/20.500.12503/29637
dc.language.isoen
dc.provenance.legacyDownloads0
dc.subjectBacteria
dc.subjectBacterial Infections and Mycoses
dc.subjectBacteriology
dc.subjectCell Anatomy
dc.subjectCell and Developmental Biology
dc.subjectCell Biology
dc.subjectComputational Biology
dc.subjectDevelopmental Biology
dc.subjectDiseases
dc.subjectGenetics
dc.subjectGenetics and Genomics
dc.subjectGenomics
dc.subjectLife Sciences
dc.subjectMedical Cell Biology
dc.subjectMedical Immunology
dc.subjectMedical Microbiology
dc.subjectMedicine and Health Sciences
dc.subjectMicrobiology
dc.subjectMolecular Genetics
dc.subjectOther Cell and Developmental Biology
dc.subjectOther Genetics and Genomics
dc.subjectNovel sRNA
dc.subjectcarbon storage regulatory system
dc.subjectE coli
dc.subjectEscherichi coli
dc.subjectsRNA
dc.subjectbacterial systems
dc.subjectglycogen biosynthesis
dc.subjecttranscription
dc.subjectCsrA
dc.subjectCsrC
dc.subjectrepeat sequences
dc.subjectbinding agents
dc.subjectgrowth
dc.subjectresponse regulator
dc.subjectcomplementation
dc.subjectin vitro
dc.subjectsynthesis
dc.subjectmutation
dc.subjectsignaling circuitry
dc.titleA Novel sRNA Member of the Carbon Storage Regulatory System of Escherichia Coli
dc.typeThesis
dc.type.materialtext
thesis.degree.departmentGraduate School of Biomedical Sciences
thesis.degree.disciplineMicrobiology and Immunology
thesis.degree.grantorUniversity of North Texas Health Science Center at Fort Worth
thesis.degree.nameMaster of Science

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