Testosterone Replacement Therapy: Role in Modulating Oxidative Stress within the Entorhinal Cortex

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dc.contributor.advisorCunningham, Rebecca L.
dc.contributor.committeeMemberJones, Harlan P.
dc.contributor.committeeMemberRomero, Steven A.
dc.creatorTajani, Ammaar H.
dc.date.accessioned2022-05-27T15:34:55Z
dc.date.available2022-05-27T15:34:55Z
dc.date.issued2021-05
dc.description.abstractSleep apnea affects approximately a quarter of all Americans. The effects of sleep apnea involve a decrease in testosterone levels, along with an increased rate of hypertension, stroke, and coronary heart disease. Chronic Intermittent Hypoxia (CIH) has also been shown to increase aging and negatively affect cognitive function, as seen with increased oxidative stress within the Entorhinal Cortex. However, it is unknown if Testosterone Replacement Therapy (TRT) can ameliorate the impact of CIH on the entorhinal cortex, which could have significant therapeutic impacts on sleep apnea related cognitive impairment. We hypothesized that TRT would mitigate protein levels of oxidative stress and inflammation, as measured by COX2, GFAP, and Calpain enzymatic activity. Banked brain tissue from young (3 month) F344/BN F1 hybrid male rats were used. Rats were separated into three treatment groups: gonadally intact, gonadectomized (GDX), and GDX with TRT. Then rats were either exposed to room air (normoxic conditions) or CIH for 8 days. Our results showed that neither TRT nor CIH impacted inflammation and oxidative stress related proteins in the entorhinal cortex. However, rats that were exposed to isoflurane anesthesia (GDX and GDX+TRT) showed neuroinflammation. Cognitive impairment has been associated with isoflurane related surgeries. However, the mechanism is unknown, though based on the data inflammation may be involved in isoflurane induced cognitive impairment in surgical patients.
dc.format.mimetypeapplication/pdf
dc.identifier.urihttps://hdl.handle.net/20.500.12503/31160
dc.language.isoen
dc.subjectentorhinal cortex
dc.subjectisoflurane
dc.subjectanesthesia
dc.subjectinflammation
dc.subjectGFAP
dc.subjectCOX2
dc.subjectCalpain
dc.subject.meshSleep Apnea Syndromes/ therapy
dc.subject.meshHormone Replacement Therapy
dc.subject.meshAging
dc.subject.meshOxidative Stress
dc.titleTestosterone Replacement Therapy: Role in Modulating Oxidative Stress within the Entorhinal Cortex
dc.typeThesis
dc.type.materialtext
thesis.degree.departmentGraduate School of Biomedical Sciences
thesis.degree.disciplineMedical Sciences Premedical Program
thesis.degree.grantorUniversity of North Texas Health Science Center at Fort Worth
thesis.degree.nameMaster of Science

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