Validation of a Y-Chromosomal Short Tandem Repeat 10-Plex for Use in Fornesic and Paternity Laboratories

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dc.contributor.advisorArthur Eisenberg
dc.contributor.committeeMemberJoseph Warren
dc.contributor.committeeMemberJohn Planz
dc.creatorJohnson, Cassie L.
dc.date.accessioned2019-08-22T21:10:08Z
dc.date.available2019-08-22T21:10:08Z
dc.date.issued2002-08-01
dc.date.submitted2014-05-13T07:24:05-07:00
dc.description.abstractJohnson, C., Validation of a Y-Chromosomal Short Tandem Repeat 10-Plex for Use in Forensic and Paternity Laboratories. Master of Science (Forensic Genetics), August, 2002. 94 pp., 6 tables, 7 figures, references, 21 titles. Y-chromosome microsatellite markers (Y-STRs) provide valuable information in cases of rape and questioned paternity, and they allow for the genetic identification of males. The present study validated a Y-STR 10-plex on the ABI Prism 3100 Genetic Analyzer for use in forensic and paternity laboratories at Orchid GeneScreen. Following optimization of the polymerase chain reaction, father-son pairs were analyzed to ensure that each pair generated identical haplotypes. The mutation rate varied between 0-0.238 (+/- 0.045, 95% confidence interval). The present study demonstrated that the 10-plex is sensitive to 0.75 ng and that female samples mixed with male samples did not interfere with Y-STR haplotyping. A population database of 525 males was developed and subsequently analyzed. Three instances of locus multiplication were observed, two at DYS19 and one at DYS435. Overall haplotype diversity was 0.996, suggesting that the 10-plex can efficiently distinguish male Y-STR profiles.
dc.format.mimetypeapplication/pdf
dc.identifier.urihttps://hdl.handle.net/20.500.12503/29124
dc.language.isoen
dc.provenance.legacyDownloads0
dc.subjectCell and Developmental Biology
dc.subjectComputational Biology
dc.subjectEquipment and Supplies
dc.subjectForensic Science and Technology
dc.subjectGenetics
dc.subjectGenetics and Genomics
dc.subjectGenetic Structures
dc.subjectHealth Information Technology
dc.subjectInvestigative Techniques
dc.subjectLife Sciences
dc.subjectMedicine and Health Sciences
dc.subjectOther Genetics and Genomics
dc.subjectValidation
dc.subjecty-chromosomal short tandem repeat
dc.subject10-plex
dc.subjectforensic laboratories
dc.subjectpaternity laboratories
dc.subjectrape
dc.subjectY-chromosome microsatellite markers
dc.subjectY-STRs
dc.subjectgenetic identification
dc.subjectmales
dc.subjectOrchid GeneScreen
dc.subjectABI Prism 3100 Genetic Analyzer
dc.subjectmutation rate
dc.subjecthaplotype diversity
dc.subjectpopulation database
dc.titleValidation of a Y-Chromosomal Short Tandem Repeat 10-Plex for Use in Fornesic and Paternity Laboratories
dc.typeProfessional Report
dc.type.materialtext
thesis.degree.departmentGraduate School of Biomedical Sciences
thesis.degree.disciplineCell Biology and Genetics
thesis.degree.grantorUniversity of North Texas Health Science Center at Fort Worth
thesis.degree.nameMaster of Science

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