Sexually Dimorphic Anxiety-Like Interoceptive Discriminative Stimuli
dc.contributor.advisor | Walls, Cleatus | |
dc.contributor.committeeMember | Downey, H. Fred | |
dc.contributor.committeeMember | Forster, Michael | |
dc.creator | Jung, Marianna E. | |
dc.date.accessioned | 2019-08-22T21:28:06Z | |
dc.date.available | 2019-08-22T21:28:06Z | |
dc.date.issued | 1997-12-01 | |
dc.date.submitted | 2013-08-27T13:28:54-07:00 | |
dc.description.abstract | Jung, Marianna E., Sexually Dimorphic Anxiety-Like Interoceptive Discriminative Stimuli. Doctor of Philosophy (Biomedical Sciences), December 1997, 150 pp, introduction, 2 chapters, discussion, bibliography, 109 titles. This study compared gender differences in the anxiogenic stimuli induced by either a GABA-A antagonist, pentylenetetrazol (PTZ) or by a 5-HT1b/2 agonist, m-chlorophenylpiperazine (m-CPP) before and during ethanol withdrawal (EW). Rats were trained to discriminate either PTZ (16mg/kg, IP) or m-CPP (1.2 mg/kg, IP) from saline in a two lever choice task for food reward. Male and female rats were gonadectomized or sham-operated, and ovariectomized (OVX) female rats were tested during replacement treatment with 17β estradiol (2.5 mg, 21 day release, sc). The dose-response for the discrimination of the interoceptive stimulus (IDS) produced by PTZ (0-16 mg/kg) or m-CPP (0 to 1.2 mg/kg) was measured under all hormonal conditions. For m-CPP trained rats, latency to first lever-press response was also tested. Results: sham and estradiol-replaced female rats had higher ED50s for discrimination of the PTZ or m-CPP IDS than intact males or OVX rats. There is a dose-related impairment of operant responding after mCPP injection. Sham and estradiol replaced OVX rats showed an increased delay to the initiation of response after m-CPP injection as compared to sham or castrated male rats or OVX rats that showed no effect at the doses tested. Rats then received a chronic ethanol diet (6.5%) for 10 days. At twelve hours of ethanol withdrawl, they were tested for lever selection after saline injection. Fewer sham female and estradiol-replaced female rats responded on the drug lever during acute EW as compared to sham male, castrated or OVX rats. In general, the anxiogenic drug lever selection of OVX rats resembled that of male rats but was restored toward that of sham female rats by estradiol replacement. Castration did not alter the response of male rats to either PTZ or mCPP. Serum β –estradiol concentrations were determined by radioimmunoassay for sham, OVX, and estradiol-replaced female rats. The concentration was significantly higher in hormone-replaced female rats than in OVX. The estradiol concentration in sham female rats showed a cyclic pattern over 4 consecutive days, but this pattern did not correlate with any difference in IDS. Blood ethanol concentration (BEC) was determined using head space gas chromatography. BEC was higher in intact female rats than in intact male rats after ethanol injection (2 g/kg, ip), but did not differ during EW. Conclusions: females produce less anxiogenic IDS in response to either GABA inhibition or 5-HT1b/2 activation, but are more impaired by m-CPP in their ability to initiate operant responses than male rats. In addition, fewer intact females developed a spontaneous IDS during EW than males which is not the result of lower BEC. Estrogen appears to play a trophic role in altering responsiveness to anxiogenic stimuli. | |
dc.format.mimetype | application/pdf | |
dc.identifier.uri | https://hdl.handle.net/20.500.12503/29354 | |
dc.language.iso | en | |
dc.provenance.legacyDownloads | 0 | |
dc.subject | Animal Sciences | |
dc.subject | Animal Structures | |
dc.subject | Behavioral Neurobiology | |
dc.subject | Behavior and Behavior Mechanisms | |
dc.subject | Biochemical and Biomolecular Engineering | |
dc.subject | Biochemistry | |
dc.subject | Biology | |
dc.subject | Biomedical | |
dc.subject | Catalysis and Reaction Engineering | |
dc.subject | Cell and Developmental Biology | |
dc.subject | Cell Biology | |
dc.subject | Chemical Actions and Uses | |
dc.subject | Chemicals and Drugs | |
dc.subject | Cognitive Neuroscience | |
dc.subject | Comparative and Laboratory Animal Medicine | |
dc.subject | Ecology and Evolutionary Biology | |
dc.subject | Life Sciences | |
dc.subject | Medical Cell Biology | |
dc.subject | Medicine and Health Sciences | |
dc.subject | Molecular and Cellular Neuroscience | |
dc.subject | Molecular Biology | |
dc.subject | Nervous System | |
dc.subject | Nervous System Diseases | |
dc.subject | Neuroscience and Neurobiology | |
dc.subject | Neurosciences | |
dc.subject | Other Neuroscience and Neurobiology | |
dc.subject | Other Nutrition | |
dc.subject | Pharmacy and Pharmaceutical Sciences | |
dc.subject | Physiological Processes | |
dc.subject | Reproductive and Urinary Physiology | |
dc.subject | Signal Processing | |
dc.subject | Structural Biology | |
dc.subject | Substance Abuse and Addiction | |
dc.subject | Systems and Communications | |
dc.subject | Systems Neuroscience | |
dc.subject | Therapeutics | |
dc.subject | Veterinary Toxicology and Pharmacology | |
dc.subject | Sexually dimorphic anxiety-like discriminative stimuli | |
dc.subject | anxiogenic stimuli | |
dc.subject | GABA-A antagonist | |
dc.subject | pentylenetetrazol | |
dc.subject | m-chlorophenylpiperazine | |
dc.subject | gonadectomize | |
dc.subject | ovariectomize | |
dc.subject | interoceptive stimulus | |
dc.subject | ethanol diet | |
dc.subject | ethanol withdrawl | |
dc.subject | lever selection | |
dc.subject | saline injection | |
dc.subject | estradiol concentration | |
dc.subject | blood ethanol concentration | |
dc.subject | GABA inhibition | |
dc.subject | 5-HT1b/2 activation | |
dc.title | Sexually Dimorphic Anxiety-Like Interoceptive Discriminative Stimuli | |
dc.type | Dissertation | |
dc.type.material | text | |
thesis.degree.department | Graduate School of Biomedical Sciences | |
thesis.degree.discipline | Biomedical Sciences | |
thesis.degree.grantor | University of North Texas Health Science Center at Fort Worth | |
thesis.degree.name | Doctor of Philosophy |
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