Novel combinatorial treatment effects on SP1 and Survivin in Ewing Sarcoma
| dc.creator | Lambring, Christoffer B. | |
| dc.creator.orcid | 0009-0003-2921-5021 (Lambring, Christoffer B.) | |
| dc.date.accessioned | 2021-04-30T14:26:06Z | |
| dc.date.available | 2021-04-30T14:26:06Z | |
| dc.date.issued | 2021 | |
| dc.description.abstract | Purpose: Ewing Sarcoma (ES) is a bone and soft tissue cancer affecting young adults and children. ES most occurs in the bones or soft tissue of the arms, legs, and pelvis. Localized ES presents with 5-year survival rate of 70%, but metastatic 5-year survival rate is between 15% and 30%. Our laboratory is interested in combination treatments using less toxic agents to induce sensitization to chemotherapy in ES. The anti-cancer activity of a Non-Steroidal Anti-Inflammatory Drug, tolfenamic acid (TA), against ES cells has been shown. TA inhibits Specificity protein 1 (Sp1) and survivin, these markers are associated with aggressive cancer cell growth and resistance to chemo/radiation therapies. This studies purpose is to evaluate the effectiveness of TA and Copper-TA (Cu-TA) to inhibit ES cell growth alongside Vincristine (VCR). Methods: Anti-proliferative activity of TA or Cu-TA and/or (VCR) against ES cell lines, TC32 and CHLA258, was evaluated using CellTiterGlo kit. Dose curves were plotted and IC50 values were determined by Sigma-Plot software. The expression of Sp1 and survivin was determined by Western bot analysis. Results: When compared to TA, Cu-TA's IC50 values were significantly less. Cu-TA inhibited the expression of Sp1 and survivin in ES cells. The combination of Cu-TA and VCR showed higher efficiency for inhibiting ES cells. Conclusions: Cu-TA may effectively sensitize certain ES cells and induce the response of chemotherapy. Studies to understand the mechanism of action of Cu-TA are underway. | |
| dc.identifier.uri | https://hdl.handle.net/20.500.12503/30479 | |
| dc.language.iso | en | |
| dc.title | Novel combinatorial treatment effects on SP1 and Survivin in Ewing Sarcoma | |
| dc.type | poster | |
| dc.type.material | text |