Identification of Unknown PCR Products Generated During STR Analysis of Bone Samples

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dc.contributor.advisorBruce Budowle
dc.creatorKoehn, Alyssa R
dc.date.accessioned2019-08-22T21:40:14Z
dc.date.available2019-08-22T21:40:14Z
dc.date.issued2013-05-01
dc.date.submitted2013-04-29T14:36:05-07:00
dc.description.abstractSTR typing of DNA extracted from bone samples exhibited additional amplified products with the PowerPlex® 16 HS Kit (Promega Corp., Madison, WI) and the Identifiler® Plus Amplification kit (Life Technologies, Foster City, CA). Microbial DNA found in soil that is co-extracted and amplified with the human DNA from bones may be the source of the artifacts seen during the STR analysis of the DNA samples. This project tested the hypothesis that the additional PCR products found in the STR analysis of DNA derived from bone samples are microbial or fungal in origin. This study has demonstrated that amplification of soil samples with an STR kit can produce artifacts, similar to those seen in the bone samples. A protocol was developed to isolate the artifact products. Attempts were made to Sequence the amplified PCR product; however, no sequencing results have been obtained, further experiments continue to attempt to generate sequencing results.
dc.format.mimetypeapplication/pdf
dc.identifier.urihttps://hdl.handle.net/20.500.12503/29500
dc.language.isoen
dc.provenance.legacyDownloads707
dc.subjectGenetics
dc.subjectGenetics and Genomics
dc.subjectMedical Sciences
dc.subjectMedicine and Health Sciences
dc.subjectForensics
dc.subjectSTR Analysis
dc.subjectBone
dc.subjectmicrobial
dc.subjectsoil
dc.subjectartifact
dc.titleIdentification of Unknown PCR Products Generated During STR Analysis of Bone Samples
dc.typeThesis
dc.type.materialtext
thesis.degree.departmentGraduate School of Biomedical Sciences
thesis.degree.disciplineForensic Genetics
thesis.degree.grantorUniversity of North Texas Health Science Center at Fort Worth
thesis.degree.nameMaster of Science

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