Role of 14-3-3σ in Corneal Epithelial Differentiation

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dc.contributor.advisorDan S. Dimitrijevich
dc.contributor.committeeMemberPorunelloor Mathew
dc.contributor.committeeMemberJulian Borejdo
dc.creatorShankardas, Jwalitha
dc.date.accessioned2019-08-22T20:54:17Z
dc.date.available2019-08-22T20:54:17Z
dc.date.issued2008-05-01
dc.date.submitted2013-10-23T07:50:06-07:00
dc.description.abstractEpithelial differentiation is a highly coordinated process that is essential for proper function of epithelia in their respective tissues. In the cornea epithelial differentiation is necessary to maintain transparency of the cornea, which is essential for vision. 14-3-3σ/stratifin is a proposed epithelial cell marker, which is up regulated in response to UV damage. This up-regulation causes the cells to arrest in G2/M phase so that DNA repair can take place. In a number of epithelial cancers 14-3-3σ has been shown to be down-regulated. The function of 14-3-3σ in the corneal epithelium and other stratified epithelial tissues other than the skin has not been studied. In the cornea are no reports of hyperplasia so it would be a good model to study the role of this protein. In this study, for the first time we have evaluated the expression of 14-3-3 family of proteins in the cornea. We have also shown the involvement of 14-3-3σ in the process of corneal epithelial differentiation in vitro using primary corneal epithelial cells and in vivo using the Er/+ mouse model. We have also been able to extend in vitro lifespan of corneal epithelial cells by down regulating 14-3-3σ expression. The down regulation also resulted in immortalization and generation of a corneal epithelial cell line. We have characterized and shown that this cell line is a suitable model to study signaling cascades involving corneal epithelial proliferation, differentiation and apoptosis. Preliminary data presented in this study also imply that ΔNp63 is an upstream regulator of 14-3-3σ expression.
dc.format.mimetypeapplication/pdf
dc.identifier.urihttps://hdl.handle.net/20.500.12503/28891
dc.language.isoen
dc.provenance.legacyDownloads0
dc.subjectCell and Developmental Biology
dc.subjectCell Biology
dc.subjectCells
dc.subjectEye Diseases
dc.subjectGenetic Phenomena
dc.subjectGenetic Processes
dc.subjectGenetics and Genomics
dc.subjectGenetic Structures
dc.subjectLife Sciences
dc.subjectMedical Cell Biology
dc.subjectMedicine and Health Sciences
dc.subjectOphthalmology
dc.subjectOptometry
dc.subjectOther Cell and Developmental Biology
dc.subjectOther Genetics and Genomics
dc.subjectSense Organs
dc.subjectVision Science
dc.subjectEpithelial differentiation
dc.subjectcornea epithelial differentiation
dc.subject14-3-3σ
dc.subjectstratifin
dc.subjectDNA repair
dc.subjectproteins
dc.subjectΔNp63
dc.titleRole of 14-3-3σ in Corneal Epithelial Differentiation
dc.typeDissertation
dc.type.materialtext
thesis.degree.departmentGraduate School of Biomedical Sciences
thesis.degree.grantorUniversity of North Texas Health Science Center at Fort Worth
thesis.degree.nameDoctor of Philosophy

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