Concordance Study of Forensic Casework Samples Using the AMPFlSTR Kit, AMPFlSTR Identifiler Kit, AMPFlSTR Profiler Plus Kit and PowerPlex 16 Kit

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dc.contributor.advisorArthur Eisenberg
dc.contributor.committeeMemberJohn Planz
dc.contributor.committeeMemberJoseph Warren
dc.creatorArmstrong, Treva L.
dc.date.accessioned2019-08-22T21:09:07Z
dc.date.available2019-08-22T21:09:07Z
dc.date.issued2002-07-01
dc.date.submitted2014-05-01T11:24:05-07:00
dc.description.abstractThe PowerPlex 16, AmpFlSTR Profiler Plus and AmpFlSTR COfiler Kits allow for the co-amplification of the amelogenin gender determining marker and the thirteen core CODIS STR loci: D3S1358, FGA, vWA, D8S1179, D21S11, D18S51, D5S818, D13S317, D7S820, D16S539, THO1, TPOX and CSF1PO. PowerPlex 16 adds the Penta D and Penta E loci and the AmpFlSTR Identifiler addes the D2S1338 and D19S433 loci. Manufactures of these systems have a suggested input DNA sample range of 0.5-2.5 ng, but have been successfully used to type samples containing less than 0.5 ng of DNA. In this study, several questions were addressed: First, “Are all four STR multiple kits concordant in their reproducibility, reliability, sensitivity and efficiency?” Second, “Is one particular STR megaplex kit more applicable to routine forensic casework?” and Third, “In a mixed DNA sample can individuals, whether male or female, be differentiated?” This paper describes a casework concordance study using adjudicated nonprobative sexual assault, mixed DNA and reference blood samples. Amplifications on all samples, were performed using the AmpFlSTR COfiler, Profiler Plus and Identifiler and PowerPlex 16 Kits and genotyping results were obtained using GeneScan and Genotyper software.
dc.format.mimetypeapplication/pdf
dc.identifier.urihttps://hdl.handle.net/20.500.12503/29110
dc.language.isoen
dc.provenance.legacyDownloads0
dc.subjectBiomedical Devices and Instrumentation
dc.subjectCell and Developmental Biology
dc.subjectCell Biology
dc.subjectCells
dc.subjectEquipment and Supplies
dc.subjectForensic Science and Technology
dc.subjectGenetics
dc.subjectGenetics and Genomics
dc.subjectGenetic Structures
dc.subjectLife Sciences
dc.subjectMedical Cell Biology
dc.subjectMedical Genetics
dc.subjectMedicine and Health Sciences
dc.subjectOther Cell and Developmental Biology
dc.subjectOther Genetics and Genomics
dc.subjectPowerPlex 16
dc.subjectAmpF/STR Profiler Plus
dc.subjectAmpF/STR Cofiler Kit
dc.subjectco-amplification
dc.subjectamelogenin gender determining marker
dc.subjectCODIS
dc.subjectSTR loci
dc.subjectPenta D
dc.subjectPenta E
dc.subjectmegaplex kit
dc.subjectGeneScan
dc.subjectGenotyper software
dc.titleConcordance Study of Forensic Casework Samples Using the AMPFlSTR Kit, AMPFlSTR Identifiler Kit, AMPFlSTR Profiler Plus Kit and PowerPlex 16 Kit
dc.typeProfessional Report
dc.type.materialtext
thesis.degree.departmentGraduate School of Biomedical Sciences
thesis.degree.disciplineCell Biology and Genetics
thesis.degree.grantorUniversity of North Texas Health Science Center at Fort Worth
thesis.degree.nameMaster of Science

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