Modulation of Astrocyte Phenotype in Response to T-cell Interaction

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dc.contributor.advisorYang, Shaohua
dc.contributor.committeeMemberSmith, Michael L.
dc.contributor.committeeMemberJin, Kunlin
dc.contributor.committeeMemberHodge, Lisa M.
dc.creatorHersh, Jessica M.
dc.date.accessioned2022-05-27T15:34:54Z
dc.date.available2022-05-27T15:34:54Z
dc.date.issued2021-05
dc.description.abstractWe determined that T-cell astrocyte interaction modulates interleukin-10 (IL-10) production from both cell types. The impact of IL-10 on astrocytes was compared to IL-10 generated from T-cell-astrocyte interactions in vitro. We demonstrated that T-cells directly interact with astrocytes to upregulate gene expression and secretion of IL-10, confirmed by elevated STAT3p/STAT3 expression in astrocytes. IL-10 increased astrocytes proliferation. In addition, IL-10 treatment and CD4+ co-culture shifts primary astrocytes toward a more energetic phenotype. These findings indicate that direct interaction of CD4+ T-cells with astrocytes, activated the IL-10 anti-inflammatory pathway, altering astrocyte phenotype, metabolism, and proliferation.
dc.format.mimetypeapplication/pdf
dc.identifier.urihttps://hdl.handle.net/20.500.12503/31150
dc.language.isoen
dc.subjectastrocytes
dc.subjectT-cells
dc.subjectCD4+
dc.subjectInterleukin-10
dc.subjectSTAT-3
dc.subjectneuroinflammation
dc.subject.meshAstrocytes
dc.subject.meshAged
dc.subject.meshIschemic Stroke
dc.subject.meshCD4-Positive-T-Lymphocytes
dc.titleModulation of Astrocyte Phenotype in Response to T-cell Interaction
dc.typeThesis
dc.type.materialtext
thesis.degree.departmentGraduate School of Biomedical Sciences
thesis.degree.disciplinePharmacology and Neuroscience
thesis.degree.grantorUniversity of North Texas Health Science Center at Fort Worth
thesis.degree.nameDoctor of Philosophy

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