Obesity Genetics: The Prevalence of DRD2, DAT1 and DBH Genes in the Obese Individual

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dc.contributor.advisorEisenberg, Arthur
dc.contributor.committeeMemberAgarwal, Neeraj
dc.contributor.committeeMemberSherman, Mark
dc.creatorDavis, Karla R.
dc.date.accessioned2019-08-22T20:14:23Z
dc.date.available2019-08-22T20:14:23Z
dc.date.issued1998-08-01
dc.date.submitted2013-12-13T10:06:04-08:00
dc.description.abstractDavis, Karla R., Obesity Genetics: The prevalence of DRD2, DAT1 and DBH Genes in the obese individual. Master of Science (Biomedical Sciences), August, 1998, 106 pp., 3 tables, 14 illustrations, reference, 44 titles. Obesity has been presented in research literature as a polygenic or multiple gene disorder. Currently, 3 genes have been associated with obesity, dopamine receptor D2 (DRD2), dopamine transporter (DAT1), and dopamine beta hydroxylase (DBH). The primary objective of this study is to analyze the DRD2, DAT1 and DBH genes to determine if a correlation exists between certain allelic variations of these 3 genes and the body mass index of obese individuals. We have developed an assay for the DRD2, DAT1 and DBH genes, utilizing polymerase chain reaction (PCR) technology. Within the DRD2 gene, 2 allelic variants have been identified, the A1 and A2 alleles. The A1 allele consists of a 310 bp fragment in which the Taq 1 restriction site has been deleted. The A2 allele consists of 180 bp fragment and a 130 bp fragment. The presence of the A1 allele after enzyme digestion has shown a strong correlation to obesity in prior studies. With respect to the DAT1 gene, a VNTR of 40 bp’s has been correlated to other disorders within the ‘reward deficiency syndrome’. The fragment length identified most often is 440 or 480 bp, with 480 as the primary fragment in obesity. The DBH gene is similar to the DRD2 in that it also contains a Taq I restriction. Two allelic variants are also identified, B1 and B2. The B1 allele contains no Taq I site and produces a 316 bp fragment while the B2 does cleave, exhibiting an 86 bp and a 230 bp fragment after enzyme digestion. The presence of one or more of the aberrant alleles could be associated with and a predisposing factor to obesity.
dc.format.mimetypeapplication/pdf
dc.identifier.urihttps://hdl.handle.net/20.500.12503/28103
dc.language.isoen
dc.provenance.legacyDownloads0
dc.subjectBiological Factors
dc.subjectCell and Developmental Biology
dc.subjectCell Biology
dc.subjectCellular and Molecular Physiology
dc.subjectDevelopmental Biology
dc.subjectGenetics
dc.subjectGenetics and Genomics
dc.subjectGenomics
dc.subjectLife Sciences
dc.subjectMedical Genetics
dc.subjectMedicine and Health Sciences
dc.subjectOther Cell and Developmental Biology
dc.subjectOther Genetics and Genomics
dc.subjectObesity genetics
dc.subjectDRD2
dc.subjectDAT1
dc.subjectDBH
dc.subjectgenes
dc.subjectdopamine receptor d2
dc.subjectdopamine transporter
dc.subjectdopamine beta hydroxylase
dc.subjectallelic variations
dc.subjectpolymerase chain reaction
dc.subjectPCR
dc.subjectTaq I
dc.subjectobese
dc.titleObesity Genetics: The Prevalence of DRD2, DAT1 and DBH Genes in the Obese Individual
dc.typeThesis
dc.type.materialtext
thesis.degree.departmentGraduate School of Biomedical Sciences
thesis.degree.disciplineBiomedical Sciences
thesis.degree.grantorUniversity of North Texas Health Science Center at Fort Worth
thesis.degree.nameMaster of Science

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