Approaches to Cloning and Identification of the Ligand for Natural Cytotoxicity Receptor NKp44

Simple item page
dc.contributor.advisorHarlan Jones
dc.contributor.committeeMemberStanley Stevens
dc.contributor.committeeMemberRaghu Krishnamoorthy
dc.creatorHorton, Nathan C.
dc.date.accessioned2019-08-22T19:50:44Z
dc.date.available2019-08-22T19:50:44Z
dc.date.issued2008-07-01
dc.date.submitted2014-02-05T06:30:05-08:00
dc.description.abstractHorton, Nathan C., Approaches to Cloning and Identification of the Ligand for the Natural Cytotoxicity Receptor, NKp44. Masters of Science (Microbiology & Immunology), July 2008, 64 pp., 22 illustrations, 37 titles. Natural Killer (NK) cells represent a specialized lymphoid population that mediate innate immune responses against tumor or virally infected cells. NK cell cytotoxicity is regulated by inhibitory and activating receptors. Activating receptors include the Natural Cytotoxicity Receptors (NCRs), 2B4, and NKG2D. The NCRs play a key role in recognition and killing of tumor cells and include the receptors NKp30, NKp46, and NKp44. The ligands for the NCRs are not yet known. NKp44 is of particular interest because it is only expressed on activated NK cells, and is implicated in increased cytotoxicity and HIV infection. To identify and clone the ligand for NKp44, a recombinant fusion protein containing the extracellular domain of NKp44 was constructed and used to identify a cell line, DB, expressing a ligand for NKp44. A directional complimentary DNA (cDNA) library was constructed from this cell line and screened by mammalian expression cloning, resulting in the isolation of several putative cDNA clones of NKp44 ligands.
dc.format.mimetypeapplication/pdf
dc.identifier.urihttps://hdl.handle.net/20.500.12503/27092
dc.language.isoen
dc.provenance.legacyDownloads0
dc.subjectCell and Developmental Biology
dc.subjectCells
dc.subjectGenetics
dc.subjectGenetics and Genomics
dc.subjectLife Sciences
dc.subjectMedical Cell Biology
dc.subjectMedical Immunology
dc.subjectMedical Microbiology
dc.subjectMedical Sciences
dc.subjectMedicine and Health Sciences
dc.subjectMolecular Genetics
dc.subjectOther Cell and Developmental Biology
dc.subjectOther Genetics and Genomics
dc.subjectCloning
dc.subjectidentification
dc.subjectligand
dc.subjectnatural cytotoxicity receptor
dc.subjectnkp44
dc.subjectnatural killer cells
dc.subjecttumor cells
dc.subjectNCR
dc.subjectdirectional complimentary DNA
dc.subjectcDNA
dc.titleApproaches to Cloning and Identification of the Ligand for Natural Cytotoxicity Receptor NKp44
dc.typeThesis
dc.type.materialtext
thesis.degree.departmentGraduate School of Biomedical Sciences
thesis.degree.disciplineMicrobiology and Immunology
thesis.degree.grantorUniversity of North Texas Health Science Center at Fort Worth
thesis.degree.nameMaster of Science

Files

Original bundle

Now showing 1 - 1 of 1
Thumbnail Image
Name:
Horton_ApproachesToCloningAnd.pdf
Size:
20.32 MB
Format:
Adobe Portable Document Format
Download

Collections

School of Biomedical Sciences
Theses and Dissertations