Combination of Small Extracellular Vesicle-Derived Annexin A2 Protein and mRNA as a Potential Predictive Biomarker for Chemotherapy Responsiveness in Aggressive Triple-Negative Breast Cancer
dc.creator | Desai, Priyanka P. | |
dc.creator | Narra, Kalyani | |
dc.creator | James, Johanna D. | |
dc.creator | Jones, Harlan P. | |
dc.creator | Tripathi, Amit K. | |
dc.creator | Vishwanatha, Jamboor K. | |
dc.creator.orcid | 0000-0002-0266-6020 (Vishwanatha, Jamboor K.) | |
dc.creator.orcid | 0000-0003-3248-0355 (Jones, Harlan P.) | |
dc.date.accessioned | 2023-02-16T14:25:40Z | |
dc.date.available | 2023-02-16T14:25:40Z | |
dc.date.issued | 2023-01-09 | |
dc.description.abstract | Small extracellular vesicles (sEVs), mainly exosomes, are nanovesicles that shed from the membrane as intraluminal vesicles of the multivesicular bodies, serve as vehicles that carry cargo influential in modulating the tumor microenvironment for the multi-step process of cancer metastasis. Annexin A2 (AnxA2), a calcium(Ca(2+))-dependent phospholipid-binding protein, is among sEV cargoes. sEV-derived AnxA2 (sEV-AnxA2) protein is involved in the process of metastasis in triple-negative breast cancer (TNBC). The objective of the current study is to determine whether sEV-AnxA2 protein and/or mRNA could be a useful biomarkers to predict the responsiveness of chemotherapy in TNBC. Removal of Immunoglobulin G (IgG) from the serum as well as using the System Bioscience's ExoQuick Ultra kit resulted in efficient sEV isolation and detection of sEV-AnxA2 protein and mRNA compared to the ultracentrifugation method. The standardized method was applied to the twenty TNBC patient sera for sEV isolation. High levels of sEV-AnxA2 protein and/or mRNA were associated with stage 3 and above in TNBC. Four patients who responded to neoadjuvant chemotherapy had high expression of AnxA2 protein and/or mRNA in sEVs, while other four who did not respond to chemotherapy had low levels of AnxA2 protein and mRNA in sEVs. Our data suggest that the sEV-AnxA2 protein and mRNA could be a combined predictive biomarker for responsiveness to chemotherapy in aggressive TNBC. | |
dc.description.sponsorship | This work was supported by awards from the National Institute of Health under awards R01CA220273 and S21MD012472 to Dr. Jamboor K. Vishwanatha. The Cryo-EM study was performed in collaboration with the Structural Biology Laboratory and the Cryo-Electron Microscopy Facility at UT Southwestern Medical Center, partially supported by grant RP170644 from the Cancer Prevention & Research Institute of Texas (CPRIT) for Cryo-EM studies. | |
dc.identifier.citation | Desai, P. P., Narra, K., James, J. D., Jones, H. P., Tripathi, A. K., & Vishwanatha, J. K. (2022). Combination of Small Extracellular Vesicle-Derived Annexin A2 Protein and mRNA as a Potential Predictive Biomarker for Chemotherapy Responsiveness in Aggressive Triple-Negative Breast Cancer. Cancers, 15(1), 212. https://doi.org/10.3390/cancers15010212 | |
dc.identifier.issn | 2072-6694 | |
dc.identifier.issue | 1 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12503/32026 | |
dc.identifier.volume | 15 | |
dc.publisher | MDPI | |
dc.relation.uri | https://doi.org/10.3390/cancers15010212 | |
dc.rights.holder | © 2022 by the authors. | |
dc.rights.license | Attribution 4.0 International (CC BY 4.0) | |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
dc.source | Cancers (Basel) | |
dc.subject | annexin A2 | |
dc.subject | biomarker | |
dc.subject | neo-adjuvant chemotherapy | |
dc.subject | small extracellular vesicles | |
dc.subject | triple-negative breast cancer | |
dc.title | Combination of Small Extracellular Vesicle-Derived Annexin A2 Protein and mRNA as a Potential Predictive Biomarker for Chemotherapy Responsiveness in Aggressive Triple-Negative Breast Cancer | |
dc.type | Article | |
dc.type.material | text |
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