ParaDNA: A Novel Instrument for Presumptive DNA Analysis

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dc.contributor.advisorWarren, Joseph E.
dc.contributor.committeeMemberAllen, Michael S.
dc.contributor.committeeMemberStaub, Rick W.
dc.contributor.committeeMemberMillar, J. Cameron
dc.creatorMoussa, Ola
dc.date.accessioned2019-10-01T20:39:30Z
dc.date.available2019-10-01T20:39:30Z
dc.date.issued2018-05
dc.description.abstractCurrent methods for human identification are time consuming and can take weeks to complete, leading to a backlog of evidence needing to be processed and a slowdown in investigations. The ParaDNA[R] Instrument is designed to address this issue using the Screening and Intelligence Systems which can detect the relative amount of DNA in an evidence item, and analyze 5 short tandem repeats (STRs) and Amelogenin, respectively [3]. The Instrument uses HyBeacons[R] which target specific STRs to identify the presence of DNA, and detect STR alleles. This is a validation of the ParaDNA[R] Screening and Intelligence Systems using saliva and blood samples to assess the sensitivity and reliability of the instrument. The data collected using the ParaDNA[R] Instrument show that it can reliably identify the relative amount of DNA in a sample, and display useful STR profiles that are 99% concordant with Qiagen Investigator 24Plex QS STR Kits.
dc.format.mimetypeapplication/pdf
dc.identifier.urihttps://hdl.handle.net/20.500.12503/29690
dc.language.isoen
dc.subjectDNA
dc.subjectForensics
dc.subjectGenetics
dc.subjectParaDNA
dc.subject.meshForensic Genetics, instrumentation
dc.subject.meshMicrosatellite Repeats
dc.subject.meshValidation Studies
dc.titleParaDNA: A Novel Instrument for Presumptive DNA Analysis
dc.typeThesis
dc.type.materialtext
thesis.degree.departmentGraduate School of Biomedical Sciences
thesis.degree.disciplineForensic Genetics
thesis.degree.grantorUniversity of North Texas Health Science Center at Fort Worth
thesis.degree.nameMaster of Science

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