Forensics, Touch DNA, Genetic Profiling, Fluorescence, DNA detection

Simple item page
dc.contributor.advisorCoble, Michael D.
dc.contributor.committeeMemberGryczynski, Zygmunt
dc.contributor.committeeMemberCihlar, Jennifer C.
dc.creatorDuque, Tiffany M.
dc.date.accessioned2024-06-04T19:40:01Z
dc.date.available2024-06-04T19:40:01Z
dc.date.issued2023-05
dc.description.abstractCurrent methods for forensic DNA typing focus on the identification and processing of bodily fluids and tissues, while touch DNA evidence has remained less used due to the inability to detect where this type of DNA is deposited. This study utilizes four DNA-binding fluorescent dyes (Diamond™ Nucleic Acid Dye, SYBR™ Green I Nucleic Acid Stain, Ethidium Bromide, and YOYO™) to detect DNA at three concentrations (100, 500, and 1000 pg/μL) and explores the interactions and effects the dye may have on subsequent genetic profiling. Genetic profiling was conducted at three time points (0, 1, and 4 weeks,) via STR amplification and capillary electrophoresis (CE). DNA was detected using all dyes and increases in fluorescence were observed in response to increases in DNA concentration or dye volume added. Most samples produced full genetic profiles, all of which were stable over the 4-week time period.
dc.format.mimetypeapplication/pdf
dc.identifier.urihttps://hdl.handle.net/20.500.12503/32827
dc.language.isoen
dc.subjectforensics
dc.subjecttouch DNA
dc.subjectgenetic profiling
dc.subjectfluorescence
dc.subjectDNA detection
dc.subject.meshFluorescent Dyes
dc.subject.meshGenetic Profile
dc.subject.meshForensice Genetics
dc.titleForensics, Touch DNA, Genetic Profiling, Fluorescence, DNA detection
dc.typeThesis
dc.type.materialtext
thesis.degree.departmentSchool of Biomedical Sciences
thesis.degree.disciplineForensic Genetics
thesis.degree.grantorUniversity of North Texas Health Science Center at Fort Worth
thesis.degree.nameMaster of Science

Files

Original bundle

Now showing 1 - 1 of 1
Thumbnail Image
Name:
2023_05_sbs_Duque, Tiffany_thesis.pdf
Size:
1.65 MB
Format:
Adobe Portable Document Format
Download

Collections

School of Biomedical Sciences
Theses and Dissertations