Establishing an STR allele frequency database for the Paraguayan population

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dc.contributor.advisorPlanz, John V.
dc.contributor.committeeMemberWarren, Joseph E.
dc.contributor.committeeMemberPhillips, Nicole R.
dc.contributor.committeeMemberCunningham, J. Thomas
dc.creatorGiuffrida, Stephanie M.
dc.date.accessioned2019-09-30T15:17:55Z
dc.date.available2019-09-30T15:17:55Z
dc.date.issued2018-05
dc.description.abstractThis project determined whether the loci included in the Athos PCR Directa para Identificacao Humana Amplification and AmpFlSTR[R] Identifiler[R] PCR Amplification Kits are suitable for establishment of an STR allele frequency database representative of the Paraguayan population. Allele frequencies for the 21 STR loci included in the Athos PCR Directa para Identificacao Humana Amplification Kit were calculated for 200 individuals in the Paraguayan population. Allele frequencies for the 15 STR loci included in the AmpFlSTR[R] Identifiler[R] PCR Amplification Kit were also calculated for 300 individuals in the Paraguayan population. Performance of these loci was determined by testing the population for Hardy-Weinberg Equilibrium, testing for Linkage Disequilibrium between the loci in the kits, and calculating Power of Discrimination, Power of Exclusion, and Mean Power of Exclusion. Comparability between the common loci included in the two PCR kits was also tested. Results of these tests determined that the Athos PCR Directa para Identificacao Humana Amplification and AmpFlSTR[R] Identifiler[R] PCR Amplification Kits are both suitable for use in an STR allele frequency database. Additionally, the common loci contained in the two PCR kits are comparable, and the populations can be combined to create one database.
dc.format.mimetypeapplication/pdf
dc.identifier.urihttps://hdl.handle.net/20.500.12503/29684
dc.language.isoen
dc.subjectallele frequency database
dc.subjectHardy-Weinberg Equilibrium
dc.subjectLinkage Equilibrium
dc.subjectParaguay
dc.subjectpopulation genetics
dc.subject.meshGene Frequency
dc.subject.meshPolymerase Chain Reaction
dc.subject.meshGenetics, Population
dc.subject.meshParaguay
dc.titleEstablishing an STR allele frequency database for the Paraguayan population
dc.typeThesis
dc.type.materialtext
thesis.degree.departmentGraduate School of Biomedical Sciences
thesis.degree.disciplineBiomedical Sciences
thesis.degree.grantorUniversity of North Texas Health Science Center at Fort Worth
thesis.degree.nameMaster of Science

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